The effect of non-traditional cooling on dog sperm cryosurvival and ability to perform the acrosome reaction

Abstract: The objective of this study was to assess cryosurvival, plasma membrane fluidity, and capability of cryopreserved dog (Canis lupus familiaris) spermatozoa, cooled to -5 °C before freezing, to perform the acrosome reaction under the effect of progesterone and calcium ionophore. In the first...

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Autores principales: Ortega-Morales,Luis D., Alcantar-Rodriguez,Alicia, Espejel,Maria C., Medrano,Alfredo
Lenguaje:English
Publicado: Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2019
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322019000200073
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Sumario:Abstract: The objective of this study was to assess cryosurvival, plasma membrane fluidity, and capability of cryopreserved dog (Canis lupus familiaris) spermatozoa, cooled to -5 °C before freezing, to perform the acrosome reaction under the effect of progesterone and calcium ionophore. In the first experiment, fresh spermatozoa diluted in Tyrode&#8217;s medium plus albumin, lactate, and pyruvate (TALP) were incubated at 38 °C in 5% CO2 in air, with progesterone or calcium ionophore added at 2, 4, and 6 h after incubation and sampled 30 min later to assess the acrosome reaction. In the second experiment, diluted sperm were packaged in plastic straws, cooled to either +5 °C or -5 °C and cryopreserved. Progressive motility, plasma membrane integrity and fluidity, capacitation status and acrosome integrity were assessed before and after freeze-thawing. After thawing, sperm were assessed, resuspended in TALP and incubated to assess the acrosome reaction. Parameters for sperm cryosurvival were similar in sperm cooled to either +5 °C or -5 °C, except in the percentage of hyper-fluid membranes which was lower (P<0.05) in sperm cooled to -5 °C. There were no differences in the percentages of frozen-thawed spermatozoa with acrosome reaction, induced by progesterone or calcium ionophore, between cooling treatments. In conclusion, cooling of dog spermatozoa to -5 °C did not improve sperm cryosurvival but had a positive effect on plasma membrane fluidity.