Cryopreservation of horse semen with a liposome and trehalose added extender

Abstract: The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA...

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Autores principales:	Medina-León,Ariadna Z., Domínguez-Mancera,Belisario, Cazalez-Penino,Nicolás, Cervantes-Acosta,Patricia, Jácome-Sosa,Edelmira, Romero-Salas,Dora, Barrientos-Morales,Manuel
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2019
Materias:	equine semen liposomes trehalose
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322019000300119
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spelling	oai:scielo:S0719-813220190003001192019-09-25Cryopreservation of horse semen with a liposome and trehalose added extenderMedina-León,Ariadna Z.Domínguez-Mancera,BelisarioCazalez-Penino,NicolásCervantes-Acosta,PatriciaJácome-Sosa,EdelmiraRomero-Salas,DoraBarrientos-Morales,Manuel equine semen liposomes trehalose Abstract: The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA 96® 2:1 v/v and transported at 22 °C to the laboratory. Before cryopreservation, the semen was diluted with INRA Freeze® to obtain the following treatments: T1) INRA Freeze® (control), T2) INRA Freeze® + liposomes, T3) INRA Freeze® + liposomes+trehalose. Data were analysed using the Kruskal Wallis test. The percentages of sperm with intact DNA were 54.5, 57.9, and 64.8% for T1, T2 and T3, respectively (P>0.05). When evaluating the acrosomal and capacitation state after filtering with Percoll®, the percentages of spermatozoa without acrosome reaction and without capacitation were 67.8, 79.2 and 68.1% in T1, T2, and T3, respectively (P>0.05), while the capacitated sperm without acrosome reaction and without capacitation was similar in T1 (47%) and T3 (32%) (P>0.05), and lower in T2 (16%) before filtering with Percoll®. The use of liposomes and liposome-trehalose did not affect on the functional status and nuclear chromatin of the equine sperm after freezing, but it did affect the percentage of capacitated sperm without acrosome reaction after selecting the thawed semen using the Percoll® gradient.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.51 n.3 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322019000300119en10.4067/S0719-81322019000300119
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	equine semen liposomes trehalose
spellingShingle	equine semen liposomes trehalose Medina-León,Ariadna Z. Domínguez-Mancera,Belisario Cazalez-Penino,Nicolás Cervantes-Acosta,Patricia Jácome-Sosa,Edelmira Romero-Salas,Dora Barrientos-Morales,Manuel Cryopreservation of horse semen with a liposome and trehalose added extender
description	Abstract: The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA 96® 2:1 v/v and transported at 22 °C to the laboratory. Before cryopreservation, the semen was diluted with INRA Freeze® to obtain the following treatments: T1) INRA Freeze® (control), T2) INRA Freeze® + liposomes, T3) INRA Freeze® + liposomes+trehalose. Data were analysed using the Kruskal Wallis test. The percentages of sperm with intact DNA were 54.5, 57.9, and 64.8% for T1, T2 and T3, respectively (P>0.05). When evaluating the acrosomal and capacitation state after filtering with Percoll®, the percentages of spermatozoa without acrosome reaction and without capacitation were 67.8, 79.2 and 68.1% in T1, T2, and T3, respectively (P>0.05), while the capacitated sperm without acrosome reaction and without capacitation was similar in T1 (47%) and T3 (32%) (P>0.05), and lower in T2 (16%) before filtering with Percoll®. The use of liposomes and liposome-trehalose did not affect on the functional status and nuclear chromatin of the equine sperm after freezing, but it did affect the percentage of capacitated sperm without acrosome reaction after selecting the thawed semen using the Percoll® gradient.
author	Medina-León,Ariadna Z. Domínguez-Mancera,Belisario Cazalez-Penino,Nicolás Cervantes-Acosta,Patricia Jácome-Sosa,Edelmira Romero-Salas,Dora Barrientos-Morales,Manuel
author_facet	Medina-León,Ariadna Z. Domínguez-Mancera,Belisario Cazalez-Penino,Nicolás Cervantes-Acosta,Patricia Jácome-Sosa,Edelmira Romero-Salas,Dora Barrientos-Morales,Manuel
author_sort	Medina-León,Ariadna Z.
title	Cryopreservation of horse semen with a liposome and trehalose added extender
title_short	Cryopreservation of horse semen with a liposome and trehalose added extender
title_full	Cryopreservation of horse semen with a liposome and trehalose added extender
title_fullStr	Cryopreservation of horse semen with a liposome and trehalose added extender
title_full_unstemmed	Cryopreservation of horse semen with a liposome and trehalose added extender
title_sort	cryopreservation of horse semen with a liposome and trehalose added extender
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322019000300119
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Cryopreservation of horse semen with a liposome and trehalose added extender

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