Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes

Abstract: The objective of this study was to compare two segregation methods to select resistant and susceptible female Pelibuey lambs infected naturally with gastrointestinal nematodes (GINs) in relation to their haematological and immunological response. For 6 months, faeces and blood samples were...

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Autores principales:	Cruz-Tamayo,Alvar, González-Garduño,Roberto, Torres-Hernández,Glafiro, Becerril-Pérez,Carlos M., Hernández-Mendo,Omar, Ramírez-Bribiesca,Jacinto Efrén, López-Arellano,María E., Vargas-Magaña,Juan J., Ojeda-Robertos,Nadia F.
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2020
Materias:	eosinophils selection IgA plasma protein
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322020000100103
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spelling	oai:scielo:S0719-813220200001001032020-01-30Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodesCruz-Tamayo,AlvarGonzález-Garduño,RobertoTorres-Hernández,GlafiroBecerril-Pérez,Carlos M.Hernández-Mendo,OmarRamírez-Bribiesca,Jacinto EfrénLópez-Arellano,María E.Vargas-Magaña,Juan J.Ojeda-Robertos,Nadia F. eosinophils selection IgA plasma protein Abstract: The objective of this study was to compare two segregation methods to select resistant and susceptible female Pelibuey lambs infected naturally with gastrointestinal nematodes (GINs) in relation to their haematological and immunological response. For 6 months, faeces and blood samples were taken fortnightly from 40 grazing 5-month-old female lambs. The lambs were classified according to two methods using faecal egg count (FEC) as a phenotypical trait. In the first (reference) method (M3SE, n = 22), resistant (RES) lambs had FEC lower than the mean - 3 standard errors, the susceptible (SUS) lambs levels higher than + 3 standard errors (n = 10) and the intermediate (INT) lambs (n = 8) were categorised by having FECs between the two values. The second method (QUM) divided the population, using quartiles, into resistant (RES; 25%), intermediate (INT; 50%), and susceptible (SUS; 25%) lambs. The agreement between both methods was estimated using the Kappa index. The packed cell volume (PCV), total plasma protein (TPP) and peripheral eosinophils (EOS) were determined for each group. Serum was used to evaluate the IgA levels. PCV and TPP values were higher (P<0.01) in the RES lambs (31.5 ± 3.4 and 6.16 ± 0.5 g/dL by QUM, respectively, and 31.5 ± 3.9 and 6.24 ± 0.49 g/dL by M3SE, respectively) than the SUS lambs (28.1 ± 4.7 and 5.94 ± 0.5 g/d, respectively, by both methods). The EOS and IgA values increased with age. M3SE and QUM were in moderate agreement (Kappa = 0.43). We concluded that the two segregation methods allowed for the identification of the same female SUS lambs, but a greater number of animals were categorised phenotypically as resistant using the M3SE method. PCV and TPP can help to identify phenotypically resistant animals.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.52 n.1 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322020000100103en10.4067/S0719-81322020000100103
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	eosinophils selection IgA plasma protein
spellingShingle	eosinophils selection IgA plasma protein Cruz-Tamayo,Alvar González-Garduño,Roberto Torres-Hernández,Glafiro Becerril-Pérez,Carlos M. Hernández-Mendo,Omar Ramírez-Bribiesca,Jacinto Efrén López-Arellano,María E. Vargas-Magaña,Juan J. Ojeda-Robertos,Nadia F. Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes
description	Abstract: The objective of this study was to compare two segregation methods to select resistant and susceptible female Pelibuey lambs infected naturally with gastrointestinal nematodes (GINs) in relation to their haematological and immunological response. For 6 months, faeces and blood samples were taken fortnightly from 40 grazing 5-month-old female lambs. The lambs were classified according to two methods using faecal egg count (FEC) as a phenotypical trait. In the first (reference) method (M3SE, n = 22), resistant (RES) lambs had FEC lower than the mean - 3 standard errors, the susceptible (SUS) lambs levels higher than + 3 standard errors (n = 10) and the intermediate (INT) lambs (n = 8) were categorised by having FECs between the two values. The second method (QUM) divided the population, using quartiles, into resistant (RES; 25%), intermediate (INT; 50%), and susceptible (SUS; 25%) lambs. The agreement between both methods was estimated using the Kappa index. The packed cell volume (PCV), total plasma protein (TPP) and peripheral eosinophils (EOS) were determined for each group. Serum was used to evaluate the IgA levels. PCV and TPP values were higher (P<0.01) in the RES lambs (31.5 ± 3.4 and 6.16 ± 0.5 g/dL by QUM, respectively, and 31.5 ± 3.9 and 6.24 ± 0.49 g/dL by M3SE, respectively) than the SUS lambs (28.1 ± 4.7 and 5.94 ± 0.5 g/d, respectively, by both methods). The EOS and IgA values increased with age. M3SE and QUM were in moderate agreement (Kappa = 0.43). We concluded that the two segregation methods allowed for the identification of the same female SUS lambs, but a greater number of animals were categorised phenotypically as resistant using the M3SE method. PCV and TPP can help to identify phenotypically resistant animals.
author	Cruz-Tamayo,Alvar González-Garduño,Roberto Torres-Hernández,Glafiro Becerril-Pérez,Carlos M. Hernández-Mendo,Omar Ramírez-Bribiesca,Jacinto Efrén López-Arellano,María E. Vargas-Magaña,Juan J. Ojeda-Robertos,Nadia F.
author_facet	Cruz-Tamayo,Alvar González-Garduño,Roberto Torres-Hernández,Glafiro Becerril-Pérez,Carlos M. Hernández-Mendo,Omar Ramírez-Bribiesca,Jacinto Efrén López-Arellano,María E. Vargas-Magaña,Juan J. Ojeda-Robertos,Nadia F.
author_sort	Cruz-Tamayo,Alvar
title	Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes
title_short	Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes
title_full	Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes
title_fullStr	Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes
title_full_unstemmed	Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes
title_sort	comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322020000100103
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Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes

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