In search of a combined brucellosis and tuberculosis vaccine for cattle

ABSTRACT: Bovine brucellosis is caused by Brucella abortus. The bacterial pathogen causes economic losses because it induces abortion in cattle. Vaccination of calves with live B. abortus strain 19 induces a certain level of protection but induces persistent antibodies against cell envelope lipopoly...

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Autores principales:	Schurig,Gerhardt G., Boyle,Stephen M., Qublan,Hamzeh Al, Sriranganathan,Nammalwar
Lenguaje:	English
Publicado:	Universidad Austral de Chile. Facultad de Ciencias Veterinarias 2021
Materias:	Brucella Mycobacterium vaccine RB51 protection
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322021000100001
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spelling	oai:scielo:S0719-813220210001000012021-02-07In search of a combined brucellosis and tuberculosis vaccine for cattleSchurig,Gerhardt G.Boyle,Stephen M.Qublan,Hamzeh AlSriranganathan,Nammalwar Brucella Mycobacterium vaccine RB51 protection ABSTRACT: Bovine brucellosis is caused by Brucella abortus. The bacterial pathogen causes economic losses because it induces abortion in cattle. Vaccination of calves with live B. abortus strain 19 induces a certain level of protection but induces persistent antibodies against cell envelope lipopolysaccharide that make it difficult to Distinguish Infected from Vaccinated Animals (DIVA). Live vaccine B. abortus strain RB51 was developed to elimínate such interfering antibodies and therefore, facilitate the differentiation of infected from vaccinated animals and help in the eradication of the disease. Vaccination with strain RB51 induces levels of protection similar to strain 19 but neither of the two vaccines give complete protection. We have been working to enhance protection induced by strain RB51 vaccine. Protective Brucella antigens can be over-expressed in strain RB51 by introducing a plasmid containing the leuB gene and the genes encoding such antigens. To avoid the expression of antibiotic resistance genes, we produced a leuB deficient strain RB51 and introduced a plasmid containing the leuB gene and the genes to be over-expressed. This new strain maintains the plasmid and has induced significantly high protection levels in mice. In addition, it allowed the construction of an RB51 vaccine strain able to express Mycobacterium bovis protective antigens so that the vaccine could protect against brucellosis and tuberculosis simultaneously.info:eu-repo/semantics/openAccessUniversidad Austral de Chile. Facultad de Ciencias VeterinariasAustral journal of veterinary sciences v.53 n.1 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322021000100001en10.4067/S0719-81322021000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Brucella Mycobacterium vaccine RB51 protection
spellingShingle	Brucella Mycobacterium vaccine RB51 protection Schurig,Gerhardt G. Boyle,Stephen M. Qublan,Hamzeh Al Sriranganathan,Nammalwar In search of a combined brucellosis and tuberculosis vaccine for cattle
description	ABSTRACT: Bovine brucellosis is caused by Brucella abortus. The bacterial pathogen causes economic losses because it induces abortion in cattle. Vaccination of calves with live B. abortus strain 19 induces a certain level of protection but induces persistent antibodies against cell envelope lipopolysaccharide that make it difficult to Distinguish Infected from Vaccinated Animals (DIVA). Live vaccine B. abortus strain RB51 was developed to elimínate such interfering antibodies and therefore, facilitate the differentiation of infected from vaccinated animals and help in the eradication of the disease. Vaccination with strain RB51 induces levels of protection similar to strain 19 but neither of the two vaccines give complete protection. We have been working to enhance protection induced by strain RB51 vaccine. Protective Brucella antigens can be over-expressed in strain RB51 by introducing a plasmid containing the leuB gene and the genes encoding such antigens. To avoid the expression of antibiotic resistance genes, we produced a leuB deficient strain RB51 and introduced a plasmid containing the leuB gene and the genes to be over-expressed. This new strain maintains the plasmid and has induced significantly high protection levels in mice. In addition, it allowed the construction of an RB51 vaccine strain able to express Mycobacterium bovis protective antigens so that the vaccine could protect against brucellosis and tuberculosis simultaneously.
author	Schurig,Gerhardt G. Boyle,Stephen M. Qublan,Hamzeh Al Sriranganathan,Nammalwar
author_facet	Schurig,Gerhardt G. Boyle,Stephen M. Qublan,Hamzeh Al Sriranganathan,Nammalwar
author_sort	Schurig,Gerhardt G.
title	In search of a combined brucellosis and tuberculosis vaccine for cattle
title_short	In search of a combined brucellosis and tuberculosis vaccine for cattle
title_full	In search of a combined brucellosis and tuberculosis vaccine for cattle
title_fullStr	In search of a combined brucellosis and tuberculosis vaccine for cattle
title_full_unstemmed	In search of a combined brucellosis and tuberculosis vaccine for cattle
title_sort	in search of a combined brucellosis and tuberculosis vaccine for cattle
publisher	Universidad Austral de Chile. Facultad de Ciencias Veterinarias
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-81322021000100001
work_keys_str_mv	AT schuriggerhardtg insearchofacombinedbrucellosisandtuberculosisvaccineforcattle AT boylestephenm insearchofacombinedbrucellosisandtuberculosisvaccineforcattle AT qublanhamzehal insearchofacombinedbrucellosisandtuberculosisvaccineforcattle AT sriranganathannammalwar insearchofacombinedbrucellosisandtuberculosisvaccineforcattle
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In search of a combined brucellosis and tuberculosis vaccine for cattle

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