In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential

Abstract A micropropagation protocol for G. pumila was developed. Young shoots were collected during the growing season (October to December 2016) from a wild population in the Villarrica Volcano area in the Araucanía Region of Chile. Nodal segments were used for in vitro initiation after testing se...

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Autores principales:	Pico-Mendoza,José, Garcia-Gonzáles,Rolando, Quiroz,Karla, Chong,Borys, Pino,Hugo, Carrasco,Basilio
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile.Facultad de Agronomía e Ingeniería Forestal 2021
Materias:	Ericaceae Gaultheria sp. in vitro rooting micropropagation plant growth regulators
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S2452-57312021000200083
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spelling	oai:scielo:S2452-573120210002000832021-09-27In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potentialPico-Mendoza,JoséGarcia-Gonzáles,RolandoQuiroz,KarlaChong,BorysPino,HugoCarrasco,Basilio Ericaceae Gaultheria sp. in vitro rooting micropropagation plant growth regulators Abstract A micropropagation protocol for G. pumila was developed. Young shoots were collected during the growing season (October to December 2016) from a wild population in the Villarrica Volcano area in the Araucanía Region of Chile. Nodal segments were used for in vitro initiation after testing several disinfection treatments with different concentrations of sodium hypochlorite. Disinfected explants were placed onto 100% WPM basal medium (WPM100) supplemented with a range of concentrations of 2-iP (2-isopentenyladenine) to evaluate the best regeneration media during in vitro culture. Disinfection with 1% sodium hypochlorite for 40 minutes, followed by a second disinfection with 2% sodium hypochlorite for 25 minutes, and cultivation on MS basal medium supplemented with 2 mg L−1 2-iP gave the highest efficiency of disinfected plants. In the propagation stage, the highest multiplication rates were obtained when 1 mg L−1 zeatin was added to the basal WPM100 medium. In vitro rooting and preacclimation were better when elongated plants were cultivated on WPM100 supplemented with 3 mg L−1 naphthalene acetic acid. This in vitro protocol could be used to propagate genotypes of this Chilean native species and is also an important tool toward its domestication and commercial use.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile.Facultad de Agronomía e Ingeniería ForestalInternational journal of agriculture and natural resources v.48 n.2 20212021-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S2452-57312021000200083en10.7764/ijanr.v48i2.2310
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ericaceae Gaultheria sp. in vitro rooting micropropagation plant growth regulators
spellingShingle	Ericaceae Gaultheria sp. in vitro rooting micropropagation plant growth regulators Pico-Mendoza,José Garcia-Gonzáles,Rolando Quiroz,Karla Chong,Borys Pino,Hugo Carrasco,Basilio In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential
description	Abstract A micropropagation protocol for G. pumila was developed. Young shoots were collected during the growing season (October to December 2016) from a wild population in the Villarrica Volcano area in the Araucanía Region of Chile. Nodal segments were used for in vitro initiation after testing several disinfection treatments with different concentrations of sodium hypochlorite. Disinfected explants were placed onto 100% WPM basal medium (WPM100) supplemented with a range of concentrations of 2-iP (2-isopentenyladenine) to evaluate the best regeneration media during in vitro culture. Disinfection with 1% sodium hypochlorite for 40 minutes, followed by a second disinfection with 2% sodium hypochlorite for 25 minutes, and cultivation on MS basal medium supplemented with 2 mg L−1 2-iP gave the highest efficiency of disinfected plants. In the propagation stage, the highest multiplication rates were obtained when 1 mg L−1 zeatin was added to the basal WPM100 medium. In vitro rooting and preacclimation were better when elongated plants were cultivated on WPM100 supplemented with 3 mg L−1 naphthalene acetic acid. This in vitro protocol could be used to propagate genotypes of this Chilean native species and is also an important tool toward its domestication and commercial use.
author	Pico-Mendoza,José Garcia-Gonzáles,Rolando Quiroz,Karla Chong,Borys Pino,Hugo Carrasco,Basilio
author_facet	Pico-Mendoza,José Garcia-Gonzáles,Rolando Quiroz,Karla Chong,Borys Pino,Hugo Carrasco,Basilio
author_sort	Pico-Mendoza,José
title	In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential
title_short	In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential
title_full	In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential
title_fullStr	In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential
title_full_unstemmed	In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential
title_sort	in vitro propagation of gaultheria pumila (l.f.) d.j. middleton (ericaceae), a chilean native berry with commercial potential
publisher	Pontificia Universidad Católica de Chile.Facultad de Agronomía e Ingeniería Forestal
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S2452-57312021000200083
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In vitro propagation of Gaultheria pumila (L.f.) D.J. Middleton (Ericaceae), a Chilean native berry with commercial potential

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