Mostrando 21 - 40 Resultados de 189 Para Buscar '"Gene targeting"', tiempo de consulta: 0.05s Limitar resultados
  1. 21
    CRISPR/Cas9-Mediated Gene Disruption Reveals the Importance of Zinc Metabolism for Fitness of the Dimorphic Fungal Pathogen <named-content content-type="genus-species">Blastomyces dermatitidis</named-content>
    “... by the relative inefficiency of traditional recombination-based gene-targeting approaches. Here, we demonstrate...”
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  2. 22
    To nick or not to nick: comparison of I-SceI single- and double-strand break-induced recombination in yeast and human cells.
    “... recombination (HR) machinery. Gene targeting is stimulated by generation of a DNA double-strand break (DSB...”
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  3. 23
    Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in <italic toggle="yes">Leishmania</italic>
    por Wen-Wei Zhang, Greg Matlashewski
    Publicado 2019
    “... of DSBs led to cell death and why the CRISPR gene-targeting efficiency is low compared with that in other...”
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  4. 24
    Dual loss of human POLQ and LIG4 abolishes random integration
    por Shinta Saito, Ryo Maeda, Noritaka Adachi
    Publicado 2017
    “...Homologous recombination mediated gene targeting is highly inefficient in human cells due to random...”
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  5. 25
    Development of a CRISPR-Cas9 System for Efficient Genome Editing of <named-content content-type="genus-species">Candida lusitaniae</named-content>
    “... is necessary for efficient gene targeting and can be successfully applied to multiple genes in both haploid...”
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  6. 26
    A comparative analysis of constitutive promoters located in adeno-associated viral vectors.
    “... gene. This assay revealed that the CMV promoter led to the lowest PIGA gene targeting efficiency...”
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  7. 27
    Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA
    “...Random off-target integration events can impair precise gene targeting and poses a safety risk...”
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  8. 28
    Pooled library screening with multiplexed Cpf1 library
    “... demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting...”
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  9. 29
    Microhomology-assisted scarless genome editing in human iPSCs
    “...Positive selection for gene targeting is a common and reliable method to generate isogenic disease...”
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  10. 30
    Phosphorylation of CENP-A on serine 7 does not control centromere function
    “.... Here, the authors use gene targeting at both endogenous CENP-A alleles and gene replacement in human...”
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  11. 31
    Importin α7 is essential for zygotic genome activation and early mouse development.
    “... the function of importin α7 by gene targeting in mice and show that it is essential for early embryonic...”
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  12. 32
    Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown.
    “...The rat is an important animal model in biomedical research, but gene targeting technology...”
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  13. 33
    I-SceI-mediated double-strand break does not increase the frequency of homologous recombination at the Dct locus in mouse embryonic stem cells.
    “... was indeed able to introduce a DSB at the Dct locus in live embryonic stem cells. However, the level of gene...”
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  14. 34
    Optimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues
    por Takahiko Matsuda, Izumi Oinuma
    Publicado 2019
    “... gene targeting was confirmed by single-cell genotyping and Western blot analysis. Several gene loci...”
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  15. 35
    Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain
    “... transforming DNA fragments, making necessary the development of more precise methods for gene targeting...”
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  16. 36
    System for stable β-estradiol-inducible gene expression in the moss Physcomitrella patens.
    “... of gene targeting and substantial genomics resources. However, current systems for controlled transgene...”
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  17. 37
    Localization of a bacterial group II intron-encoded protein in eukaryotic nuclear splicing-related cell compartments.
    “... in developing this group II intron gene targeting technology for use in eukaryotes, but nuclear genomes present...”
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  18. 38
    A cotransformation system of the unicellular red alga Cyanidioschyzon merolae with blasticidin S deaminase and chloramphenicol acetyltransferase selectable markers
    “... cellular and genomic architecture. In addition, procedures for genetic modifications, such as gene...”
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  19. 39
    Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
    “..., gene targeting, cloning, and regenerative medicine. However, the majority of attempts to establish ESC...”
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  20. 40
    Downregulation of Endothelin Receptor B Contributes to Defective B Cell Lymphopoiesis in Trisomy 21 Pluripotent Stem Cells
    “... of the EDNRB pathway in B cell differentiation was obtained through CRISPR/Cas9 gene targeting in disomic...”
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