Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulence
The first antimicrobial resistance global report on surveillance of the World Health Organization (WHO) in 2014 pointed out the need to deal with the problem under a “One Health” approach which focusses on the interactions between humans, animals and their environments, in order to enhance health an...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | text (thesis) |
| Lenguaje: | eng |
| Publicado: |
Universidad de La Rioja (España)
2020
|
| Acceso en línea: | https://dialnet.unirioja.es/servlet/oaites?codigo=267450 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai-TES0000022942 |
|---|---|
| record_format |
dspace |
| institution |
DialNet |
| collection |
DialNet |
| language |
eng |
| description |
The first antimicrobial resistance global report on surveillance of the World Health Organization (WHO) in 2014 pointed out the need to deal with the problem under a “One Health” approach which focusses on the interactions between humans, animals and their environments, in order to enhance health and welfare. Staphylococcal species (both coagulase-positive (CoPS) and -negative (CoNS)) are normal colonizers of skin and mucous of healthy humans and animals, but also opportunistic pathogens, that can cause skin and soft tissues infections, mastitis, food intoxication, bacteraemia etc. This thesis aimed to analyse the diversity of staphylococcal species in different niches, including wild and farm animals destined for human consumption, animal-derived food and humans, to determine the antimicrobial resistance phenotype/genotype of recovered isolates, and to evaluate the occurrence of livestock-associated lineages among S. aureus isolates.
The first chapter studied the molecular epidemiology of staphylococcal species in nasal and/or faecal samples of non-conventional food animals such as wild boar and horses in Spain. A great diversity of staphylococcal species was observed in wild boar, being S. aureus (76.1%) and S. hyicus (16.4%) the predominant CoPS, and S. sciuri (39.7%) and S. xylosus (13%) the predominant CoNS. In horses, the predominant species were S. aureus (37%), S. delphini (21%) and S. sciuri (21%). Most isolates recovered from wild boar were pan susceptible (73.1% of CoPS and 77.6% of CoNS). However unusual genes involved in macrolide, lincosamide and phenicol resistance were detected among CoNS. Regarding horses’ isolates, 88.7% of CoPS were pan susceptible while 48.6% of CoNS showed resistance to at least one of the antimicrobial agents. A multidrug resistance phenotype (MDR) was only observed among CoNS from both animal species. The predominant S. aureus lineage in wild boar was t3750-ST2328/CC133 (29.4%) and one isolate of the livestock-associated methicillin resistant S. aureus lineage (LA-MRSACC398) (t011-ST398) was identified. S. aureus lineage ST1640 (61.7% of S. aureus), is associated with horses for the first time in this study. All S. aureus isolates detected from horses, except those of lineage ST1460, carried the genes encoding the equid-adapted leukocidin (LukPQ) and the blocker of equine complement system activation (eqSCIN).
The second chapter focussed on staphylococci from common food-producing animals from two countries with different antibiotic use policy (calve, lamb, goat (Spain); cow and chicken (Senegal)). In Spain, the prevalence of staphylococci was higher in lamb (54%) and goat (50%) than in cow (21%) samples. In Senegal, staphylococci prevalence was higher in cow (26.8%) than in chicken (3%). In Spain, the most frequent lineage was CC133 while the ST291 was dominant in Senegal isolates. Six species were identified among CoNS in each country, with prevalent species being S. sciuri and S. simulans. CoPS isolates from Spain were mostly pan susceptible, while those from Senegal were mostly penicillin resistant. In both countries, a relatively high percentage of CoNS isolates (32.5% (Senegal), 47% (Spain)) was resistant to at least one of the antimicrobials tested. Resistance to penicillin, tetracycline and SXT was observed among CoNS isolates of both countries. Additionally, resistance to methicillin, streptomycin, clindamycin, erythromycin, chloramphenicol and tobramycin was observed among Spain isolates. The Panton-Valentine Leukocidin (PVL) encoding genes were present in isolates of both countries, and the human virulent clone USA300 was identified among Spain isolates.
In the chapters three and four, the lineage CC398 was screened for pig-derived food S. aureus isolates on one hand, and among patients’ blood culture S. aureus isolates from Spanish hospitals located in regions with different pig density degrees, on the other hand (multicentre study).
CC398 was the predominant lineage among S. aureus isolates of pig products (64.1%; n=23). The prevalence of MRSA-CC398 was 20.8%, observing differences in products with skin (76.5% (ear/snout)) and without skin (3.3% (fillet), 14.8% (chopped meat)); this clone was mostly typed as t011. All MRSA-CC398 isolates were mecA-positive, tetracycline resistant (tet(M) and tet(K)+/- tet(L)) and scn-negative. 82.6% were MDR and 17.4% harboured virulence genes. Two methicillin susceptible S. aureus (MSSA)-CC398 isolates were detected; both being resistant to penicillin and erythromycin/clindamycininducible (erm(T) gene). They were typed as t5452 and were scn-positive.
Concerning the clinical isolates, the prevalence of CC398 isolates among S. aureus was 4.3%. MSSA-CC398 represented 90.2% of total CC398 isolates, corresponding to 5.2% of MSSA and 3.9% of S. aureus. t571 and t1451 were the predominant spa-types among MSSA-CC398 isolates. Resistance to erythromycin/clindamycin-inducible, mediated by erm(T) gene, was only observed among MSSA-CC398 isolates (72.9%). All MSSA-CC398 isolates, but three, were scn-positive. No obvious correlation was observed between MSSA-CC398 prevalence at hospital level and the pig density degree in surrounding areas. LA-MRSA-CC398 and LA-MRSA-CC1 were detected in hospitals located in high pig density areas. |
| author2 |
Torres Manrique, Carmen (null) |
| author_facet |
Torres Manrique, Carmen (null) Mama, Olouwafemi Mistourath |
| format |
text (thesis) |
| author |
Mama, Olouwafemi Mistourath |
| spellingShingle |
Mama, Olouwafemi Mistourath Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulence |
| author_sort |
Mama, Olouwafemi Mistourath |
| title |
Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulence |
| title_short |
Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulence |
| title_full |
Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulence |
| title_fullStr |
Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulence |
| title_full_unstemmed |
Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulence |
| title_sort |
staphylococcus spp. from animals intended for human consumption, animalderived food and humans in spain and senegal: genetic lineages, antibioresistance and virulence |
| publisher |
Universidad de La Rioja (España) |
| publishDate |
2020 |
| url |
https://dialnet.unirioja.es/servlet/oaites?codigo=267450 |
| work_keys_str_mv |
AT mamaolouwafemimistourath staphylococcussppfromanimalsintendedforhumanconsumptionanimalderivedfoodandhumansinspainandsenegalgeneticlineagesantibioresistanceandvirulence |
| _version_ |
1718346686356520960 |
| spelling |
oai-TES00000229422020-04-23Staphylococcus spp. from animals intended for human consumption, animalderived food and humans in Spain and Senegal: genetic lineages, antibioresistance and virulenceMama, Olouwafemi MistourathThe first antimicrobial resistance global report on surveillance of the World Health Organization (WHO) in 2014 pointed out the need to deal with the problem under a “One Health” approach which focusses on the interactions between humans, animals and their environments, in order to enhance health and welfare. Staphylococcal species (both coagulase-positive (CoPS) and -negative (CoNS)) are normal colonizers of skin and mucous of healthy humans and animals, but also opportunistic pathogens, that can cause skin and soft tissues infections, mastitis, food intoxication, bacteraemia etc. This thesis aimed to analyse the diversity of staphylococcal species in different niches, including wild and farm animals destined for human consumption, animal-derived food and humans, to determine the antimicrobial resistance phenotype/genotype of recovered isolates, and to evaluate the occurrence of livestock-associated lineages among S. aureus isolates. The first chapter studied the molecular epidemiology of staphylococcal species in nasal and/or faecal samples of non-conventional food animals such as wild boar and horses in Spain. A great diversity of staphylococcal species was observed in wild boar, being S. aureus (76.1%) and S. hyicus (16.4%) the predominant CoPS, and S. sciuri (39.7%) and S. xylosus (13%) the predominant CoNS. In horses, the predominant species were S. aureus (37%), S. delphini (21%) and S. sciuri (21%). Most isolates recovered from wild boar were pan susceptible (73.1% of CoPS and 77.6% of CoNS). However unusual genes involved in macrolide, lincosamide and phenicol resistance were detected among CoNS. Regarding horses’ isolates, 88.7% of CoPS were pan susceptible while 48.6% of CoNS showed resistance to at least one of the antimicrobial agents. A multidrug resistance phenotype (MDR) was only observed among CoNS from both animal species. The predominant S. aureus lineage in wild boar was t3750-ST2328/CC133 (29.4%) and one isolate of the livestock-associated methicillin resistant S. aureus lineage (LA-MRSACC398) (t011-ST398) was identified. S. aureus lineage ST1640 (61.7% of S. aureus), is associated with horses for the first time in this study. All S. aureus isolates detected from horses, except those of lineage ST1460, carried the genes encoding the equid-adapted leukocidin (LukPQ) and the blocker of equine complement system activation (eqSCIN). The second chapter focussed on staphylococci from common food-producing animals from two countries with different antibiotic use policy (calve, lamb, goat (Spain); cow and chicken (Senegal)). In Spain, the prevalence of staphylococci was higher in lamb (54%) and goat (50%) than in cow (21%) samples. In Senegal, staphylococci prevalence was higher in cow (26.8%) than in chicken (3%). In Spain, the most frequent lineage was CC133 while the ST291 was dominant in Senegal isolates. Six species were identified among CoNS in each country, with prevalent species being S. sciuri and S. simulans. CoPS isolates from Spain were mostly pan susceptible, while those from Senegal were mostly penicillin resistant. In both countries, a relatively high percentage of CoNS isolates (32.5% (Senegal), 47% (Spain)) was resistant to at least one of the antimicrobials tested. Resistance to penicillin, tetracycline and SXT was observed among CoNS isolates of both countries. Additionally, resistance to methicillin, streptomycin, clindamycin, erythromycin, chloramphenicol and tobramycin was observed among Spain isolates. The Panton-Valentine Leukocidin (PVL) encoding genes were present in isolates of both countries, and the human virulent clone USA300 was identified among Spain isolates. In the chapters three and four, the lineage CC398 was screened for pig-derived food S. aureus isolates on one hand, and among patients’ blood culture S. aureus isolates from Spanish hospitals located in regions with different pig density degrees, on the other hand (multicentre study). CC398 was the predominant lineage among S. aureus isolates of pig products (64.1%; n=23). The prevalence of MRSA-CC398 was 20.8%, observing differences in products with skin (76.5% (ear/snout)) and without skin (3.3% (fillet), 14.8% (chopped meat)); this clone was mostly typed as t011. All MRSA-CC398 isolates were mecA-positive, tetracycline resistant (tet(M) and tet(K)+/- tet(L)) and scn-negative. 82.6% were MDR and 17.4% harboured virulence genes. Two methicillin susceptible S. aureus (MSSA)-CC398 isolates were detected; both being resistant to penicillin and erythromycin/clindamycininducible (erm(T) gene). They were typed as t5452 and were scn-positive. Concerning the clinical isolates, the prevalence of CC398 isolates among S. aureus was 4.3%. MSSA-CC398 represented 90.2% of total CC398 isolates, corresponding to 5.2% of MSSA and 3.9% of S. aureus. t571 and t1451 were the predominant spa-types among MSSA-CC398 isolates. Resistance to erythromycin/clindamycin-inducible, mediated by erm(T) gene, was only observed among MSSA-CC398 isolates (72.9%). All MSSA-CC398 isolates, but three, were scn-positive. No obvious correlation was observed between MSSA-CC398 prevalence at hospital level and the pig density degree in surrounding areas. LA-MRSA-CC398 and LA-MRSA-CC1 were detected in hospitals located in high pig density areas.El primer informe global de la Organización Mundial de la Salud (OMS) sobre la situación de la resistencia a antibióticos, publicado en 2014, destacaba la necesidad de abordar ese problema con un enfoque “One Health”, centrándose en las interacciones entre los humanos, los animales y el medio ambiente. Las especies del género Staphylococcus, tanto coagulasa-positivo (SCoP) como coagulasa-negativo (SCoN), son comensales de piel y mucosas de personas y animales sanos, siendo a su vez patógenos oportunistas que pueden causar infecciones de piel y partes blandas, mastitis, intoxicaciones alimentarias, bacteriemias, etc. El objetivo de esta tesis fue analizar en primer lugar la diversidad de especies estafilocócicas en distintos nichos ecológicos incluyendo los animales salvajes y los de granja destinados al consumo humano, los animales de producción y las personas. Posteriormente, determinar el fenotipo/genotipo de resistencia a antibióticos de los aislados detectados, y evaluar la prevalencia de las líneas asociadas al ganado entre los aislados de S. aureus. En el primer capítulo, se estudió la epidemiología molecular de Staphylococcus spp. en especies animales destinadas al consumo humano minoritarias como son los jabalíes y los caballos en España. Se observó una gran diversidad estafilocócica en muestras nasales de jabalíes, siendo S. aureus (76.1%) y S. hyicus (16.4%) las especies predominantes entre los SCoP, y S. sciuri (39.7%) y S. xylosus (13%) las predominantes entre los SCoN. En los caballos, las especies predominantes fueron: S. aureus (37%), S. delphini (21%) y S. sciuri (21%). La mayoría de los aislados de jabalíes fueron pan sensibles (73,1% de los SCoP y 77,6% de los SCoN). Sin embargo, se detectaron entre los aislados SCoN, genes inusuales codificantes de la resistencia a macrólidos, lincosamides y fenicoles. En cuanto a los aislados de caballos, un 88,7% de los SCoP fueron pan sensibles mientras que un 48,6% de los SCoN mostraron resistencia a al menos un antibiótico testado. Se observaron fenotipos de multirresistencia solo en aislados SCoN en ambas especies animales. Entre los S. aureus de jabalíes, el linaje predominante fue el t3750-ST2328/CC133 (29,4%). También se detectó un aislado t011 del clon asociado a ganado SARM-CC398. ST1640 fue la línea genética más frecuente entre los S. aureus de caballos (61,7%). Todos los aislados S. aureus de caballos, excepto los del linaje ST1640 portaban los genes codificantes de la leucocidina específica de équidos (LukPQ) y del bloqueador de la activación del sistema complementario equino (eqSCIN). El segundo capítulo se centró en estafilococos de animales de producción de dos países con diferentes políticas de uso de antibióticos (ternero, cordero y cabra (España); vaca y pollo (Senegal)). En España, la prevalencia de estafilococos fue más elevada en muestras de ovino (54%) y caprino (50%), en comparación con las de vacuno (21%). En Senegal, se detectó una prevalencia mucho más alta en vacuno (26,8%) que en pollo (3%). La línea genética de S. aureus más frecuente en los aislados de España, fue el CC133 mientras que el ST291 fue el más abundante entre los aislados de Senegal. Se identificaron seis especies SCoN en cada uno de los países, siendo S. sciuri y S. simulans las más frecuentes en ambos países. Los aislados SCoP fueron mayoritariamente pan sensibles (España) o resistentes a penicilina (Senegal). En ambos países, un porcentaje relativamente elevado de los aislados SCoN presentaron resistencia a al menos uno de los antibióticos testados (32,5% (Senegal) y 47% (España)). Entre los SCoN de ambos países se detectaron aislados resistentes a penicilina, tetraciclina y SXT. Además, se observó resistencia a meticilina, estreptomicina, clindamicina, eritromicina, cloranfenicol y tobramicina entre los aislados de España. En aislados de ambos países, se detectaron los genes codificantes de la leucocidina de Panton-Valentine y se identificó el clon USA300 entre los aislados de España. En los capítulos tres y cuatro, se analizó la presencia del linaje CC398, por un lado entre aislados S. aureus de productos derivados de cerdo, y por otro lado entre aislados S. aureus de hemocultivos de pacientes de hospitales españoles (estudio multicéntrico) situados en provincias con diferentes niveles de densidad porcina. El linaje CC398 fue predominante entre los aislados S. aureus de productos derivados de cerdo (64,1%; n=23). La prevalencia de S. aureus resistente a meticilina (SARM)-CC398 fue del 20,8% (en productos con piel (oreja/morro, 76,6%); y sin piel (filete, 3,3%; carne picada, 14,8%); siendo este clon mayoritariamente asociado al spa-tipo t011. Todos los aislados MRSA-CC398 fueron mecA-positivos, tetraciclina-resistentes (tet(M) y tet(K)+/- tet(L)) y scn-negativo. El 82,6% de estos aislados fueron multirresistentes y el 17,4% portaban genes de virulencia. Se detectaron dos aislados sensibles a meticilina (SASM)-CC398. Ambos aislados fueron resistentes a penicilina y eritromicina/clindamicina-inducible (erm(T)), scn-positivos y tipados como t5452. En cuanto a los aislados clínicos, el 4,3% de los aislados S. aureus pertenecían al linaje CC398, siendo el 90,2% de ellos SASM, lo que representa un 5,2% y 3,9% de los aislados SASM y S. aureus, respectivamente. Los spa-tipos t571 y t1451 fueron predominantes entre los aislados SASM-CC398. La resistencia a eritromicina/clindamicina-inducible mediada por el gen erm(T) fue observada solo en aislados SASM-CC398 (72,9%). Todos los aislados SASM-CC398, excepto tres, fueron scn-positivos. No se observó una relación clara entre la prevalencia de SASM-CC398 en los hospitales y el nivel de densidad porcina de la zona correspondiente. Los aislados LA-SARM-CC398 fueron infrecuentes y detectados en hospitales situados en provincias de alta densidad porcina.Universidad de La Rioja (España)Torres Manrique, Carmen (null)2020text (thesis)application/pdfhttps://dialnet.unirioja.es/servlet/oaites?codigo=267450engLICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI |