Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

Given the practical limitations of solid matrix-based protocols in organoid culture, Yumiko Hirokawa et al. assess the ability of low-concentration Matrigel conditions to promote intestinal organoid growth. Their results suggest that a low-viscosity culture system can improve live cell yield compare...

Full description

Saved in:
Bibliographic Details
Main Authors: Yumiko Hirokawa, Jordan Clarke, Michelle Palmieri, Tao Tan, Dmitri Mouradov, Shan Li, Cong Lin, Fuqiang Li, Huijuan Luo, Kui Wu, Maree Faux, Chin Wee Tan, Margaret Lee, Grace Gard, Peter Gibbs, Antony W. Burgess, Oliver M. Sieber
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
Biology (General)
QH301-705.5
Online Access:https://doaj.org/article/006bc56a251a435dbc12018d048e3d9b
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Given the practical limitations of solid matrix-based protocols in organoid culture, Yumiko Hirokawa et al. assess the ability of low-concentration Matrigel conditions to promote intestinal organoid growth. Their results suggest that a low-viscosity culture system can improve live cell yield compared to the existing dome method, while maintaining similar morphology, and represents a useful approach for high-throughput applications of organoids.

Similar Items