MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers

Abstract The Fanconi Anemia (FA) pathway is essential for human cells to maintain genomic integrity following DNA damage. This pathway is involved in repairing damaged DNA through homologous recombination. Cancers with a defective FA pathway are expected to be more sensitive to cross-link based ther...

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Autores principales:	Wenrui Duan, Shirley Tang, Li Gao, Kathleen Dotts, Andrew Fink, Arjun Kalvala, Brittany Aguila, Qi-En Wang, Miguel A. Villalona-Calero
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00e5274edafd4aabbc69514504bfd27d
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spelling	oai:doaj.org-article:00e5274edafd4aabbc69514504bfd27d2021-12-02T15:52:59ZMiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers10.1038/s41598-021-83884-92045-2322https://doaj.org/article/00e5274edafd4aabbc69514504bfd27d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83884-9https://doaj.org/toc/2045-2322Abstract The Fanconi Anemia (FA) pathway is essential for human cells to maintain genomic integrity following DNA damage. This pathway is involved in repairing damaged DNA through homologous recombination. Cancers with a defective FA pathway are expected to be more sensitive to cross-link based therapy or PARP inhibitors. To evaluate downstream effectors of the FA pathway, we studied the expression of 734 different micro RNAs (miRNA) using NanoString nCounter miRNA array in two FA defective lung cancer cells and matched control cells, along with two lung tumors and matched non-tumor tissue samples that were deficient in the FA pathway. Selected miRNA expression was validated with real-time PCR analysis. Among 734 different miRNAs, a cluster of microRNAs were found to be up-regulated including an important cancer related micro RNA, miR-200C. MiRNA-200C has been reported as a negative regulator of epithelial-mesenchymal transition (EMT) and inhibits cell migration and invasion by promoting the upregulation of E-cadherin through targeting ZEB1 and ZEB2 transcription factors. miRNA-200C was increased in the FA defective lung cancers as compared to controls. AmpliSeq analysis showed significant reduction in ZEB1 and ZEB2 mRNA expression. Our findings indicate the miRNA-200C potentially play a very important role in FA pathway downstream regulation.Wenrui DuanShirley TangLi GaoKathleen DottsAndrew FinkArjun KalvalaBrittany AguilaQi-En WangMiguel A. Villalona-CaleroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenrui Duan Shirley Tang Li Gao Kathleen Dotts Andrew Fink Arjun Kalvala Brittany Aguila Qi-En Wang Miguel A. Villalona-Calero MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers
description	Abstract The Fanconi Anemia (FA) pathway is essential for human cells to maintain genomic integrity following DNA damage. This pathway is involved in repairing damaged DNA through homologous recombination. Cancers with a defective FA pathway are expected to be more sensitive to cross-link based therapy or PARP inhibitors. To evaluate downstream effectors of the FA pathway, we studied the expression of 734 different micro RNAs (miRNA) using NanoString nCounter miRNA array in two FA defective lung cancer cells and matched control cells, along with two lung tumors and matched non-tumor tissue samples that were deficient in the FA pathway. Selected miRNA expression was validated with real-time PCR analysis. Among 734 different miRNAs, a cluster of microRNAs were found to be up-regulated including an important cancer related micro RNA, miR-200C. MiRNA-200C has been reported as a negative regulator of epithelial-mesenchymal transition (EMT) and inhibits cell migration and invasion by promoting the upregulation of E-cadherin through targeting ZEB1 and ZEB2 transcription factors. miRNA-200C was increased in the FA defective lung cancers as compared to controls. AmpliSeq analysis showed significant reduction in ZEB1 and ZEB2 mRNA expression. Our findings indicate the miRNA-200C potentially play a very important role in FA pathway downstream regulation.
format	article
author	Wenrui Duan Shirley Tang Li Gao Kathleen Dotts Andrew Fink Arjun Kalvala Brittany Aguila Qi-En Wang Miguel A. Villalona-Calero
author_facet	Wenrui Duan Shirley Tang Li Gao Kathleen Dotts Andrew Fink Arjun Kalvala Brittany Aguila Qi-En Wang Miguel A. Villalona-Calero
author_sort	Wenrui Duan
title	MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers
title_short	MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers
title_full	MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers
title_fullStr	MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers
title_full_unstemmed	MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers
title_sort	mirna-200c expression in fanconi anemia pathway functionally deficient lung cancers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/00e5274edafd4aabbc69514504bfd27d
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MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers

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