Hypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence.
'Taishanzaoxia' fruit rapid softening and dehiscence during ripening stage and this process is very sensitive to endogenous ethylene. In this study, we cloned five ethylene signal transcription factors (ZMdEIL1, ZMdEIL2, ZMdEIL3, ZMdERF1 and ZMdERF2) and one functional gene, ZMdPG1, encodi...
Guardado en:
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2013
|
Materias: | |
Acceso en línea: | https://doaj.org/article/017b33b71f0946b2a4c10d8c4f02e4ff |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:017b33b71f0946b2a4c10d8c4f02e4ff |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:017b33b71f0946b2a4c10d8c4f02e4ff2021-11-18T07:52:42ZHypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence.1932-620310.1371/journal.pone.0058745https://doaj.org/article/017b33b71f0946b2a4c10d8c4f02e4ff2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23527016/?tool=EBIhttps://doaj.org/toc/1932-6203'Taishanzaoxia' fruit rapid softening and dehiscence during ripening stage and this process is very sensitive to endogenous ethylene. In this study, we cloned five ethylene signal transcription factors (ZMdEIL1, ZMdEIL2, ZMdEIL3, ZMdERF1 and ZMdERF2) and one functional gene, ZMdPG1, encoding polygalacturonase that could loose the cell connection which associated with fruit firmness decrease and fruit dehiscence to illustrate the reasons for this specific fruit phenotypic and physiological changes. Expression analysis showed that ZMdERF1 and ZMdEIL2 transcription were more abundant in 'Taishanzaoxia' softening fruit and dehiscent fruit and their expression was inhibited by an ethylene inhibitor 1-methylcyclopropene. Therefore, ZMdERF1 and ZMdEIL2 expression were responses to endogenous ethylene and associated with fruit softening and dehiscence. ZMdPG1 expression was induced when fruit softening and dehiscence but this induction can be blocked by 1-MCP, indicating that ZMdPG1 was essential for fruit softening and dehiscence and its expression was mediated by the endogenously occurred ethylene. ZMdPG1 overexpression in Arabidopsis led to silique early dehiscence while suppressing ZMdPG1 expression by antisense ZMdPG1 prevented silique naturally opening. The result also suggested that ZMdPG1 related with the connection between cells that contributed to fruit softening and dehiscence. ZMdERF1 was more closely related with ethylene signaling but it was not directly regulated the ZMdPG1, which might be regulated by the synergic pattern of ethylene transcription factors because of both the ZMdERF1 and ZMdERF2 could interact with ZMdEIL2.Min LiYanmin ZhangZongying ZhangXiaohao JiRui ZhangDaliang LiuLiping GaoJing ZhangBiao WangYusen WuShujing WuXiaoliu ChenShouqian FengXuesen ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58745 (2013) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Min Li Yanmin Zhang Zongying Zhang Xiaohao Ji Rui Zhang Daliang Liu Liping Gao Jing Zhang Biao Wang Yusen Wu Shujing Wu Xiaoliu Chen Shouqian Feng Xuesen Chen Hypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence. |
description |
'Taishanzaoxia' fruit rapid softening and dehiscence during ripening stage and this process is very sensitive to endogenous ethylene. In this study, we cloned five ethylene signal transcription factors (ZMdEIL1, ZMdEIL2, ZMdEIL3, ZMdERF1 and ZMdERF2) and one functional gene, ZMdPG1, encoding polygalacturonase that could loose the cell connection which associated with fruit firmness decrease and fruit dehiscence to illustrate the reasons for this specific fruit phenotypic and physiological changes. Expression analysis showed that ZMdERF1 and ZMdEIL2 transcription were more abundant in 'Taishanzaoxia' softening fruit and dehiscent fruit and their expression was inhibited by an ethylene inhibitor 1-methylcyclopropene. Therefore, ZMdERF1 and ZMdEIL2 expression were responses to endogenous ethylene and associated with fruit softening and dehiscence. ZMdPG1 expression was induced when fruit softening and dehiscence but this induction can be blocked by 1-MCP, indicating that ZMdPG1 was essential for fruit softening and dehiscence and its expression was mediated by the endogenously occurred ethylene. ZMdPG1 overexpression in Arabidopsis led to silique early dehiscence while suppressing ZMdPG1 expression by antisense ZMdPG1 prevented silique naturally opening. The result also suggested that ZMdPG1 related with the connection between cells that contributed to fruit softening and dehiscence. ZMdERF1 was more closely related with ethylene signaling but it was not directly regulated the ZMdPG1, which might be regulated by the synergic pattern of ethylene transcription factors because of both the ZMdERF1 and ZMdERF2 could interact with ZMdEIL2. |
format |
article |
author |
Min Li Yanmin Zhang Zongying Zhang Xiaohao Ji Rui Zhang Daliang Liu Liping Gao Jing Zhang Biao Wang Yusen Wu Shujing Wu Xiaoliu Chen Shouqian Feng Xuesen Chen |
author_facet |
Min Li Yanmin Zhang Zongying Zhang Xiaohao Ji Rui Zhang Daliang Liu Liping Gao Jing Zhang Biao Wang Yusen Wu Shujing Wu Xiaoliu Chen Shouqian Feng Xuesen Chen |
author_sort |
Min Li |
title |
Hypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence. |
title_short |
Hypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence. |
title_full |
Hypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence. |
title_fullStr |
Hypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence. |
title_full_unstemmed |
Hypersensitive ethylene signaling and ZMdPG1 expression lead to fruit softening and dehiscence. |
title_sort |
hypersensitive ethylene signaling and zmdpg1 expression lead to fruit softening and dehiscence. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doaj.org/article/017b33b71f0946b2a4c10d8c4f02e4ff |
work_keys_str_mv |
AT minli hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT yanminzhang hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT zongyingzhang hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT xiaohaoji hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT ruizhang hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT daliangliu hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT lipinggao hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT jingzhang hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT biaowang hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT yusenwu hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT shujingwu hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT xiaoliuchen hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT shouqianfeng hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence AT xuesenchen hypersensitiveethylenesignalingandzmdpg1expressionleadtofruitsofteninganddehiscence |
_version_ |
1718422805452685312 |