Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain

The high-yielding production of pharmaceutically significant secondary metabolites in filamentous fungi is obtained by random mutagenesis; such changes may be associated with shifts in the metabolism of polyamines. We have previously shown that, in the <i>Acremonium chrysogenum</i> cepha...

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Autores principales:	Alexander A. Zhgun, Mikhail A. Eldarov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyamines filamentous fungi biosynthesis of secondary metabolites <i>Acremonium chrysogenum</i> cephalosporin C Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/02c53477827742fe8593a9c78549d77c
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spelling	oai:doaj.org-article:02c53477827742fe8593a9c78549d77c2021-11-11T18:36:22ZPolyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain10.3390/molecules262166361420-3049https://doaj.org/article/02c53477827742fe8593a9c78549d77c2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6636https://doaj.org/toc/1420-3049The high-yielding production of pharmaceutically significant secondary metabolites in filamentous fungi is obtained by random mutagenesis; such changes may be associated with shifts in the metabolism of polyamines. We have previously shown that, in the <i>Acremonium chrysogenum</i> cephalosporin C high-yielding strain (HY), the content of endogenous polyamines increased by four- to five-fold. Other studies have shown that the addition of exogenous polyamines can increase the production of target secondary metabolites in highly active fungal producers, in particular, increase the biosynthesis of β-lactams in the <i>Penicillium chrysogenum</i> Wis 54–1255 strain, an improved producer of penicillin G. In the current study, we demonstrate that the introduction of exogenous polyamines, such as spermidine or 1,3-diaminopropane, to <i>A. chrysogenum</i> wild-type (WT) and HY strains, leads to an increase in colony germination and morphological changes in a complete agar medium. The addition of 5 mM polyamines during fermentation increases the production of cephalosporin C in the <i>A. chrysogenum</i> HY strain by 15–20% and upregulates genes belonging to the beta-lactam biosynthetic cluster. The data obtained indicate the intersection of the metabolisms of polyamines and beta-lactams in <i>A. chrysogenum</i> and are important for the construction of improved producers of secondary metabolites in filamentous fungi.Alexander A. ZhgunMikhail A. EldarovMDPI AGarticlepolyaminesfilamentous fungibiosynthesis of secondary metabolites<i>Acremonium chrysogenum</i>cephalosporin COrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6636, p 6636 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyamines filamentous fungi biosynthesis of secondary metabolites <i>Acremonium chrysogenum</i> cephalosporin C Organic chemistry QD241-441
spellingShingle	polyamines filamentous fungi biosynthesis of secondary metabolites <i>Acremonium chrysogenum</i> cephalosporin C Organic chemistry QD241-441 Alexander A. Zhgun Mikhail A. Eldarov Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain
description	The high-yielding production of pharmaceutically significant secondary metabolites in filamentous fungi is obtained by random mutagenesis; such changes may be associated with shifts in the metabolism of polyamines. We have previously shown that, in the <i>Acremonium chrysogenum</i> cephalosporin C high-yielding strain (HY), the content of endogenous polyamines increased by four- to five-fold. Other studies have shown that the addition of exogenous polyamines can increase the production of target secondary metabolites in highly active fungal producers, in particular, increase the biosynthesis of β-lactams in the <i>Penicillium chrysogenum</i> Wis 54–1255 strain, an improved producer of penicillin G. In the current study, we demonstrate that the introduction of exogenous polyamines, such as spermidine or 1,3-diaminopropane, to <i>A. chrysogenum</i> wild-type (WT) and HY strains, leads to an increase in colony germination and morphological changes in a complete agar medium. The addition of 5 mM polyamines during fermentation increases the production of cephalosporin C in the <i>A. chrysogenum</i> HY strain by 15–20% and upregulates genes belonging to the beta-lactam biosynthetic cluster. The data obtained indicate the intersection of the metabolisms of polyamines and beta-lactams in <i>A. chrysogenum</i> and are important for the construction of improved producers of secondary metabolites in filamentous fungi.
format	article
author	Alexander A. Zhgun Mikhail A. Eldarov
author_facet	Alexander A. Zhgun Mikhail A. Eldarov
author_sort	Alexander A. Zhgun
title	Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain
title_short	Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain
title_full	Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain
title_fullStr	Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain
title_full_unstemmed	Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain
title_sort	polyamines upregulate cephalosporin c production and expression of β-lactam biosynthetic genes in high-yielding <i>acremonium chrysogenum</i> strain
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/02c53477827742fe8593a9c78549d77c
work_keys_str_mv	AT alexanderazhgun polyaminesupregulatecephalosporincproductionandexpressionofblactambiosyntheticgenesinhighyieldingiacremoniumchrysogenumistrain AT mikhailaeldarov polyaminesupregulatecephalosporincproductionandexpressionofblactambiosyntheticgenesinhighyieldingiacremoniumchrysogenumistrain
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Polyamines Upregulate Cephalosporin C Production and Expression of β-Lactam Biosynthetic Genes in High-Yielding <i>Acremonium chrysogenum</i> Strain

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