Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway

Background. Intervertebral disc is responsible for absorbing and transmitting mechanical compression. Under physiological conditions, the peripheral annulus fibrosus (AF) cells are subjected to different magnitudes of transverse mechanical stretch depending on the swelling of the central nucleus pul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li Ning, Lei Gao, Fan Zhang, Xiaoxiao Li, Tingting Wang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/030d7908d53c49b295f683041c6a8b73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:030d7908d53c49b295f683041c6a8b73
record_format	dspace
spelling	oai:doaj.org-article:030d7908d53c49b295f683041c6a8b732021-11-29T00:56:18ZMechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway2314-614110.1155/2021/5321121https://doaj.org/article/030d7908d53c49b295f683041c6a8b732021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/5321121https://doaj.org/toc/2314-6141Background. Intervertebral disc is responsible for absorbing and transmitting mechanical compression. Under physiological conditions, the peripheral annulus fibrosus (AF) cells are subjected to different magnitudes of transverse mechanical stretch depending on the swelling of the central nucleus pulposus tissue. However, the biological behavior of AF cells under mechanical stretch is not well studied. Objective. This study was performed to study the effects of mechanical tension on AF cell senescence and the potential signaling transduction pathway. Methods. Rat AF cells were made to experience different magnitudes of mechanical stretch (2% elongation and 20% elongation for 4 hours every day at 1 Hz) in a 10-day experiment period. The inhibitor RKI-1447 of the Rho-associated coiled-coil–containing protein kinases (ROCK) was added along with culture medium to investigate its role. Cell proliferation, cell cycle, telomerase activity, and expression of senescence markers (p16 and p53) were analyzed. Results. We found that 20% elongation significantly decreased cell proliferation, promoted G0/G1 cell cycle arrest, decreased telomerase activity, and upregulated mRNA/protein expression of p16 and p53. Moreover, the inhibitor RKI-1447 partly resisted effects of 20% elongation on these parameters of cell senescence. Conclusion. High mechanical stretch obviously induces AF cell senescence through the RhoA/ROCK pathway. This study provides us a deeper understanding on the AF cell’s behavior under mechanical stretch.Li NingLei GaoFan ZhangXiaoxiao LiTingting WangHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Li Ning Lei Gao Fan Zhang Xiaoxiao Li Tingting Wang Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway
description	Background. Intervertebral disc is responsible for absorbing and transmitting mechanical compression. Under physiological conditions, the peripheral annulus fibrosus (AF) cells are subjected to different magnitudes of transverse mechanical stretch depending on the swelling of the central nucleus pulposus tissue. However, the biological behavior of AF cells under mechanical stretch is not well studied. Objective. This study was performed to study the effects of mechanical tension on AF cell senescence and the potential signaling transduction pathway. Methods. Rat AF cells were made to experience different magnitudes of mechanical stretch (2% elongation and 20% elongation for 4 hours every day at 1 Hz) in a 10-day experiment period. The inhibitor RKI-1447 of the Rho-associated coiled-coil–containing protein kinases (ROCK) was added along with culture medium to investigate its role. Cell proliferation, cell cycle, telomerase activity, and expression of senescence markers (p16 and p53) were analyzed. Results. We found that 20% elongation significantly decreased cell proliferation, promoted G0/G1 cell cycle arrest, decreased telomerase activity, and upregulated mRNA/protein expression of p16 and p53. Moreover, the inhibitor RKI-1447 partly resisted effects of 20% elongation on these parameters of cell senescence. Conclusion. High mechanical stretch obviously induces AF cell senescence through the RhoA/ROCK pathway. This study provides us a deeper understanding on the AF cell’s behavior under mechanical stretch.
format	article
author	Li Ning Lei Gao Fan Zhang Xiaoxiao Li Tingting Wang
author_facet	Li Ning Lei Gao Fan Zhang Xiaoxiao Li Tingting Wang
author_sort	Li Ning
title	Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway
title_short	Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway
title_full	Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway
title_fullStr	Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway
title_full_unstemmed	Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway
title_sort	mechanical stretch induces annulus fibrosus cell senescence through activation of the rhoa/rock pathway
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/030d7908d53c49b295f683041c6a8b73
work_keys_str_mv	AT lining mechanicalstretchinducesannulusfibrosuscellsenescencethroughactivationoftherhoarockpathway AT leigao mechanicalstretchinducesannulusfibrosuscellsenescencethroughactivationoftherhoarockpathway AT fanzhang mechanicalstretchinducesannulusfibrosuscellsenescencethroughactivationoftherhoarockpathway AT xiaoxiaoli mechanicalstretchinducesannulusfibrosuscellsenescencethroughactivationoftherhoarockpathway AT tingtingwang mechanicalstretchinducesannulusfibrosuscellsenescencethroughactivationoftherhoarockpathway
_version_	1718407686972768256

Mechanical Stretch Induces Annulus Fibrosus Cell Senescence through Activation of the RhoA/ROCK Pathway

Ejemplares similares