Identification of a membrane-less compartment regulating invadosome function and motility

Abstract Depletion of liprin-α1, ERC1 or LL5 scaffolds inhibits extracellular matrix degradation by invasive cells. These proteins co-accumulate near invadosomes in NIH-Src cells, identifying a novel invadosome–associated compartment distinct from the core and adhesion ring of invadosomes. Depletion...

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Autores principales:	Kristyna Sala, Andrea Raimondi, Diletta Tonoli, Carlo Tacchetti, Ivan de Curtis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/03f68b9343d147fab5c3d0fb2c1ff904
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spelling	oai:doaj.org-article:03f68b9343d147fab5c3d0fb2c1ff9042021-12-02T15:08:07ZIdentification of a membrane-less compartment regulating invadosome function and motility10.1038/s41598-018-19447-22045-2322https://doaj.org/article/03f68b9343d147fab5c3d0fb2c1ff9042018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19447-2https://doaj.org/toc/2045-2322Abstract Depletion of liprin-α1, ERC1 or LL5 scaffolds inhibits extracellular matrix degradation by invasive cells. These proteins co-accumulate near invadosomes in NIH-Src cells, identifying a novel invadosome–associated compartment distinct from the core and adhesion ring of invadosomes. Depletion of either protein perturbs the organization of invadosomes without influencing the recruitment of MT1-MMP metalloprotease. Liprin-α1 is not required for de novo formation of invadosomes after their disassembly by microtubules and Src inhibitors, while its depletion inhibits invadosome motility, thus affecting matrix degradation. Fluorescence recovery after photobleaching shows that the invadosome–associated compartment is dynamic, while correlative light immunoelectron microscopy identifies bona fide membrane–free invadosome–associated regions enriched in liprin-α1, which is virtually excluded from the invadosome core. The results indicate that liprin-α1, LL5 and ERC1 define a novel dynamic membrane-less compartment that regulates matrix degradation by affecting invadosome motility.Kristyna SalaAndrea RaimondiDiletta TonoliCarlo TacchettiIvan de CurtisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristyna Sala Andrea Raimondi Diletta Tonoli Carlo Tacchetti Ivan de Curtis Identification of a membrane-less compartment regulating invadosome function and motility
description	Abstract Depletion of liprin-α1, ERC1 or LL5 scaffolds inhibits extracellular matrix degradation by invasive cells. These proteins co-accumulate near invadosomes in NIH-Src cells, identifying a novel invadosome–associated compartment distinct from the core and adhesion ring of invadosomes. Depletion of either protein perturbs the organization of invadosomes without influencing the recruitment of MT1-MMP metalloprotease. Liprin-α1 is not required for de novo formation of invadosomes after their disassembly by microtubules and Src inhibitors, while its depletion inhibits invadosome motility, thus affecting matrix degradation. Fluorescence recovery after photobleaching shows that the invadosome–associated compartment is dynamic, while correlative light immunoelectron microscopy identifies bona fide membrane–free invadosome–associated regions enriched in liprin-α1, which is virtually excluded from the invadosome core. The results indicate that liprin-α1, LL5 and ERC1 define a novel dynamic membrane-less compartment that regulates matrix degradation by affecting invadosome motility.
format	article
author	Kristyna Sala Andrea Raimondi Diletta Tonoli Carlo Tacchetti Ivan de Curtis
author_facet	Kristyna Sala Andrea Raimondi Diletta Tonoli Carlo Tacchetti Ivan de Curtis
author_sort	Kristyna Sala
title	Identification of a membrane-less compartment regulating invadosome function and motility
title_short	Identification of a membrane-less compartment regulating invadosome function and motility
title_full	Identification of a membrane-less compartment regulating invadosome function and motility
title_fullStr	Identification of a membrane-less compartment regulating invadosome function and motility
title_full_unstemmed	Identification of a membrane-less compartment regulating invadosome function and motility
title_sort	identification of a membrane-less compartment regulating invadosome function and motility
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/03f68b9343d147fab5c3d0fb2c1ff904
work_keys_str_mv	AT kristynasala identificationofamembranelesscompartmentregulatinginvadosomefunctionandmotility AT andrearaimondi identificationofamembranelesscompartmentregulatinginvadosomefunctionandmotility AT dilettatonoli identificationofamembranelesscompartmentregulatinginvadosomefunctionandmotility AT carlotacchetti identificationofamembranelesscompartmentregulatinginvadosomefunctionandmotility AT ivandecurtis identificationofamembranelesscompartmentregulatinginvadosomefunctionandmotility
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Identification of a membrane-less compartment regulating invadosome function and motility

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