Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells

Abstract The human papillomavirus (HPV) plays a central role in cervical carcinogenesis and its oncogene E7 is essential in this process. We showed here that E7 abrogated the G1 cell cycle checkpoint under hypoxia and analyzed key cell cycle related proteins for their potential role in this process....

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Autores principales:	Hanxiang Chen, Qishu Zhang, Lijun Qiao, Xueli Fan, Weifang Zhang, Weiming Zhao, Jason J. Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/04b85512866f468bb2551c1476b8588a
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spelling	oai:doaj.org-article:04b85512866f468bb2551c1476b8588a2021-12-02T11:40:51ZCdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells10.1038/s41598-017-03060-w2045-2322https://doaj.org/article/04b85512866f468bb2551c1476b8588a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03060-whttps://doaj.org/toc/2045-2322Abstract The human papillomavirus (HPV) plays a central role in cervical carcinogenesis and its oncogene E7 is essential in this process. We showed here that E7 abrogated the G1 cell cycle checkpoint under hypoxia and analyzed key cell cycle related proteins for their potential role in this process. To further explore the mechanism by which E7 bypasses hypoxia-induced G1 arrest, we applied a proteomic approach and used mass spectrometry to search for proteins that are differentially expressed in E7 expressing cells under hypoxia. Among differentially expressed proteins identified, Cdc6 is a DNA replication initiation factor and exhibits oncogenic activities when overexpressed. We have recently demonstrated that Cdc6 was required for E7-induced re-replication. Significantly, here we showed that Cdc6 played a role in E7-mediated G1 checkpoint abrogation under hypoxic condition, and the function could possibly be independent from its role in DNA replication initiation. This study uncovered a new function of Cdc6 in regulating cell cycle progression and has important implications in HPV-associated cancers.Hanxiang ChenQishu ZhangLijun QiaoXueli FanWeifang ZhangWeiming ZhaoJason J. ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hanxiang Chen Qishu Zhang Lijun Qiao Xueli Fan Weifang Zhang Weiming Zhao Jason J. Chen Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells
description	Abstract The human papillomavirus (HPV) plays a central role in cervical carcinogenesis and its oncogene E7 is essential in this process. We showed here that E7 abrogated the G1 cell cycle checkpoint under hypoxia and analyzed key cell cycle related proteins for their potential role in this process. To further explore the mechanism by which E7 bypasses hypoxia-induced G1 arrest, we applied a proteomic approach and used mass spectrometry to search for proteins that are differentially expressed in E7 expressing cells under hypoxia. Among differentially expressed proteins identified, Cdc6 is a DNA replication initiation factor and exhibits oncogenic activities when overexpressed. We have recently demonstrated that Cdc6 was required for E7-induced re-replication. Significantly, here we showed that Cdc6 played a role in E7-mediated G1 checkpoint abrogation under hypoxic condition, and the function could possibly be independent from its role in DNA replication initiation. This study uncovered a new function of Cdc6 in regulating cell cycle progression and has important implications in HPV-associated cancers.
format	article
author	Hanxiang Chen Qishu Zhang Lijun Qiao Xueli Fan Weifang Zhang Weiming Zhao Jason J. Chen
author_facet	Hanxiang Chen Qishu Zhang Lijun Qiao Xueli Fan Weifang Zhang Weiming Zhao Jason J. Chen
author_sort	Hanxiang Chen
title	Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells
title_short	Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells
title_full	Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells
title_fullStr	Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells
title_full_unstemmed	Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells
title_sort	cdc6 contributes to abrogating the g1 checkpoint under hypoxic conditions in hpv e7 expressing cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/04b85512866f468bb2551c1476b8588a
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Cdc6 contributes to abrogating the G1 checkpoint under hypoxic conditions in HPV E7 expressing cells

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