In vivo analysis of the Notch receptor S1 cleavage.

In vivo analysis of the Notch receptor S1 cleavage.

A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear an...

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Autores principales: Robert J Lake, Lisa M Grimm, Alexey Veraksa, Andrew Banos, Spyros Artavanis-Tsakonas
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2009
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Acceso en línea:https://doaj.org/article/05d2fdc75ee043be924eac48fa9137ac
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spelling oai:doaj.org-article:05d2fdc75ee043be924eac48fa9137ac2021-11-25T06:20:52ZIn vivo analysis of the Notch receptor S1 cleavage.1932-620310.1371/journal.pone.0006728https://doaj.org/article/05d2fdc75ee043be924eac48fa9137ac2009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19701455/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.Robert J LakeLisa M GrimmAlexey VeraksaAndrew BanosSpyros Artavanis-TsakonasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 8, p e6728 (2009)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Robert J Lake
Lisa M Grimm
Alexey Veraksa
Andrew Banos
Spyros Artavanis-Tsakonas
In vivo analysis of the Notch receptor S1 cleavage.
description A ligand-independent cleavage (S1) in the extracellular domain of the mammalian Notch receptor results in what is considered to be the canonical heterodimeric form of Notch on the cell surface. The in vivo consequences and significance of this cleavage on Drosophila Notch signaling remain unclear and contradictory. We determined the cleavage site in Drosophila and examined its in vivo function by a transgenic analysis of receptors that cannot be cleaved. Our results demonstrate a correlation between loss of cleavage and loss of in vivo function of the Notch receptor, supporting the notion that S1 cleavage is an in vivo mechanism of Notch signal control.
format article
author Robert J Lake
Lisa M Grimm
Alexey Veraksa
Andrew Banos
Spyros Artavanis-Tsakonas
author_facet Robert J Lake
Lisa M Grimm
Alexey Veraksa
Andrew Banos
Spyros Artavanis-Tsakonas
author_sort Robert J Lake
title In vivo analysis of the Notch receptor S1 cleavage.
title_short In vivo analysis of the Notch receptor S1 cleavage.
title_full In vivo analysis of the Notch receptor S1 cleavage.
title_fullStr In vivo analysis of the Notch receptor S1 cleavage.
title_full_unstemmed In vivo analysis of the Notch receptor S1 cleavage.
title_sort in vivo analysis of the notch receptor s1 cleavage.
publisher Public Library of Science (PLoS)
publishDate 2009
url https://doaj.org/article/05d2fdc75ee043be924eac48fa9137ac
work_keys_str_mv AT robertjlake invivoanalysisofthenotchreceptors1cleavage
AT lisamgrimm invivoanalysisofthenotchreceptors1cleavage
AT alexeyveraksa invivoanalysisofthenotchreceptors1cleavage
AT andrewbanos invivoanalysisofthenotchreceptors1cleavage
AT spyrosartavanistsakonas invivoanalysisofthenotchreceptors1cleavage
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