Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils

Abstract Fluorescence of thioflavin T (ThT) is a proven tool for amyloid fibrils study. The correct model of ThT binding to fibrils is crucial to clarify amyloid fibrils structure and mechanism of their formation. Although there are convincing evidences that ThT has molecular rotor nature, implying...

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Autores principales:	Anna I. Sulatskaya, Andrey V. Lavysh, Alexander A. Maskevich, Irina M. Kuznetsova, Konstantin K. Turoverov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/06f32f88a9e24f93b7d6feefa91a0255
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spelling	oai:doaj.org-article:06f32f88a9e24f93b7d6feefa91a02552021-12-02T15:05:23ZThioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils10.1038/s41598-017-02237-72045-2322https://doaj.org/article/06f32f88a9e24f93b7d6feefa91a02552017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02237-7https://doaj.org/toc/2045-2322Abstract Fluorescence of thioflavin T (ThT) is a proven tool for amyloid fibrils study. The correct model of ThT binding to fibrils is crucial to clarify amyloid fibrils structure and mechanism of their formation. Although there are convincing evidences that ThT has molecular rotor nature, implying it’s binding to fibrils in monomer form, speculations concerning ThT binding to fibrils in aggregated forms appear in literature so far. The elaborated approach for fluorescence intensity correction on the inner filter effects applied to ThT aqueous solutions with a wide range of concentration allowed characterizing ThT excimers fluorescence and showing its difference from that of ThT bound to fibrils. Obtained results experimentally prove the monomer model of ThT binding to amyloid fibrils and demonstrate wide capacity of the used approach in the spectroscopy of other fluorescent dyes for examination of concentration self-quenching and deformation of fluorescence spectra, dye molecules interaction, dimers and excimers formation.Anna I. SulatskayaAndrey V. LavyshAlexander A. MaskevichIrina M. KuznetsovaKonstantin K. TuroverovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna I. Sulatskaya Andrey V. Lavysh Alexander A. Maskevich Irina M. Kuznetsova Konstantin K. Turoverov Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils
description	Abstract Fluorescence of thioflavin T (ThT) is a proven tool for amyloid fibrils study. The correct model of ThT binding to fibrils is crucial to clarify amyloid fibrils structure and mechanism of their formation. Although there are convincing evidences that ThT has molecular rotor nature, implying it’s binding to fibrils in monomer form, speculations concerning ThT binding to fibrils in aggregated forms appear in literature so far. The elaborated approach for fluorescence intensity correction on the inner filter effects applied to ThT aqueous solutions with a wide range of concentration allowed characterizing ThT excimers fluorescence and showing its difference from that of ThT bound to fibrils. Obtained results experimentally prove the monomer model of ThT binding to amyloid fibrils and demonstrate wide capacity of the used approach in the spectroscopy of other fluorescent dyes for examination of concentration self-quenching and deformation of fluorescence spectra, dye molecules interaction, dimers and excimers formation.
format	article
author	Anna I. Sulatskaya Andrey V. Lavysh Alexander A. Maskevich Irina M. Kuznetsova Konstantin K. Turoverov
author_facet	Anna I. Sulatskaya Andrey V. Lavysh Alexander A. Maskevich Irina M. Kuznetsova Konstantin K. Turoverov
author_sort	Anna I. Sulatskaya
title	Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils
title_short	Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils
title_full	Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils
title_fullStr	Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils
title_full_unstemmed	Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils
title_sort	thioflavin t fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/06f32f88a9e24f93b7d6feefa91a0255
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Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils

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