Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray

Abstract X-ray irradiation of high Z elements causes photoelectric effects that include the release of Auger electrons that can induce localized DNA breaks. We have previously established a tumor spheroid-based assay that used gadolinium containing mesoporous silica nanoparticles and synchrotron-gen...

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Autores principales:	Yuya Higashi, Kotaro Matsumoto, Hiroyuki Saitoh, Ayumi Shiro, Yue Ma, Mathilde Laird, Shanmugavel Chinnathambi, Albane Birault, Tan Le Hoang Doan, Ryo Yasuda, Toshiki Tajima, Tetsuya Kawachi, Fuyuhiko Tamanoi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0774ae605b3341aab8bffe43ed7857d6
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spelling	oai:doaj.org-article:0774ae605b3341aab8bffe43ed7857d62021-12-02T16:14:03ZIodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray10.1038/s41598-021-93429-92045-2322https://doaj.org/article/0774ae605b3341aab8bffe43ed7857d62021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93429-9https://doaj.org/toc/2045-2322Abstract X-ray irradiation of high Z elements causes photoelectric effects that include the release of Auger electrons that can induce localized DNA breaks. We have previously established a tumor spheroid-based assay that used gadolinium containing mesoporous silica nanoparticles and synchrotron-generated monochromatic X-rays. In this work, we focused on iodine and synthesized iodine-containing porous organosilica (IPO) nanoparticles. IPO were loaded onto tumor spheroids and the spheroids were irradiated with 33.2 keV monochromatic X-ray. After incubation in CO2 incubator, destruction of tumor spheroids was observed which was accompanied by apoptosis induction, as determined by the TUNEL assay. By employing the γH2AX assay, we detected double strand DNA cleavages immediately after the irradiation. These results suggest that IPO first generate double strand DNA breaks upon X-ray irradiation followed by apoptosis induction of cancer cells. Use of three different monochromatic X-rays having energy levels of 33.0, 33.2 and 33.4 keV as well as X-rays with 0.1 keV energy intervals showed that the optimum effect of all three events (spheroid destruction, apoptosis induction and generation of double strand DNA breaks) occurred with a 33.2 keV monochromatic X-ray. These results uncover the preferential effect of K-edge energy X-ray for tumor spheroid destruction mediated by iodine containing nanoparticles.Yuya HigashiKotaro MatsumotoHiroyuki SaitohAyumi ShiroYue MaMathilde LairdShanmugavel ChinnathambiAlbane BiraultTan Le Hoang DoanRyo YasudaToshiki TajimaTetsuya KawachiFuyuhiko TamanoiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuya Higashi Kotaro Matsumoto Hiroyuki Saitoh Ayumi Shiro Yue Ma Mathilde Laird Shanmugavel Chinnathambi Albane Birault Tan Le Hoang Doan Ryo Yasuda Toshiki Tajima Tetsuya Kawachi Fuyuhiko Tamanoi Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray
description	Abstract X-ray irradiation of high Z elements causes photoelectric effects that include the release of Auger electrons that can induce localized DNA breaks. We have previously established a tumor spheroid-based assay that used gadolinium containing mesoporous silica nanoparticles and synchrotron-generated monochromatic X-rays. In this work, we focused on iodine and synthesized iodine-containing porous organosilica (IPO) nanoparticles. IPO were loaded onto tumor spheroids and the spheroids were irradiated with 33.2 keV monochromatic X-ray. After incubation in CO2 incubator, destruction of tumor spheroids was observed which was accompanied by apoptosis induction, as determined by the TUNEL assay. By employing the γH2AX assay, we detected double strand DNA cleavages immediately after the irradiation. These results suggest that IPO first generate double strand DNA breaks upon X-ray irradiation followed by apoptosis induction of cancer cells. Use of three different monochromatic X-rays having energy levels of 33.0, 33.2 and 33.4 keV as well as X-rays with 0.1 keV energy intervals showed that the optimum effect of all three events (spheroid destruction, apoptosis induction and generation of double strand DNA breaks) occurred with a 33.2 keV monochromatic X-ray. These results uncover the preferential effect of K-edge energy X-ray for tumor spheroid destruction mediated by iodine containing nanoparticles.
format	article
author	Yuya Higashi Kotaro Matsumoto Hiroyuki Saitoh Ayumi Shiro Yue Ma Mathilde Laird Shanmugavel Chinnathambi Albane Birault Tan Le Hoang Doan Ryo Yasuda Toshiki Tajima Tetsuya Kawachi Fuyuhiko Tamanoi
author_facet	Yuya Higashi Kotaro Matsumoto Hiroyuki Saitoh Ayumi Shiro Yue Ma Mathilde Laird Shanmugavel Chinnathambi Albane Birault Tan Le Hoang Doan Ryo Yasuda Toshiki Tajima Tetsuya Kawachi Fuyuhiko Tamanoi
author_sort	Yuya Higashi
title	Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray
title_short	Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray
title_full	Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray
title_fullStr	Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray
title_full_unstemmed	Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray
title_sort	iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic x-ray irradiation: dna breaks and k-edge energy x-ray
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0774ae605b3341aab8bffe43ed7857d6
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Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray

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