Genome-scale metabolic network validation of Shewanella oneidensis using transposon insertion frequency analysis.
Transposon mutagenesis, in combination with parallel sequencing, is becoming a powerful tool for en-masse mutant analysis. A probability generating function was used to explain observed miniHimar transposon insertion patterns, and gene essentiality calls were made by transposon insertion frequency a...
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Auteurs principaux: | , , , , , , , |
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Format: | article |
Langue: | EN |
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Public Library of Science (PLoS)
2014
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Accès en ligne: | https://doaj.org/article/07abc409acf04af58d95ec660176f4de |
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