Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments

For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information. Here authors provide cryo-EM structures of actin filaments with N-1-pyrene conjugated to cysteine 374 a...

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Autores principales: Steven Z. Chou, Thomas D. Pollard
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/07da98c81c624041b587e3c353b40141
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