Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments

Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments

For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information. Here authors provide cryo-EM structures of actin filaments with N-1-pyrene conjugated to cysteine 374 a...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Steven Z. Chou, Thomas D. Pollard
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/07da98c81c624041b587e3c353b40141
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:07da98c81c624041b587e3c353b40141
record_format dspace
spelling oai:doaj.org-article:07da98c81c624041b587e3c353b401412021-12-02T14:40:41ZCryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments10.1038/s41467-020-19762-12041-1723https://doaj.org/article/07da98c81c624041b587e3c353b401412020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19762-1https://doaj.org/toc/2041-1723For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information. Here authors provide cryo-EM structures of actin filaments with N-1-pyrene conjugated to cysteine 374 and either ADP or ADP-phosphate in the active site.Steven Z. ChouThomas D. PollardNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Steven Z. Chou
Thomas D. Pollard
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
description For almost forty years, N-(1-pyrene) iodoacetamide has been used to label actin at C374, but the mechanisms of the fluorescence changes are still unknown due to the lack of structural information. Here authors provide cryo-EM structures of actin filaments with N-1-pyrene conjugated to cysteine 374 and either ADP or ADP-phosphate in the active site.
format article
author Steven Z. Chou
Thomas D. Pollard
author_facet Steven Z. Chou
Thomas D. Pollard
author_sort Steven Z. Chou
title Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
title_short Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
title_full Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
title_fullStr Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
title_full_unstemmed Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
title_sort cryo-electron microscopy structures of pyrene-labeled adp-pi- and adp-actin filaments
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/07da98c81c624041b587e3c353b40141
work_keys_str_mv AT stevenzchou cryoelectronmicroscopystructuresofpyrenelabeledadppiandadpactinfilaments
AT thomasdpollard cryoelectronmicroscopystructuresofpyrenelabeledadppiandadpactinfilaments
_version_ 1718390153215475712

Ejemplares similares