High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM

Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods....

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Autores principales:	Mark A. Herzik, Mengyu Wu, Gabriel C. Lander
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/082a304c2b0a4f3b94de0ccbd7867848
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spelling	oai:doaj.org-article:082a304c2b0a4f3b94de0ccbd78678482021-12-02T16:58:01ZHigh-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM10.1038/s41467-019-08991-82041-1723https://doaj.org/article/082a304c2b0a4f3b94de0ccbd78678482019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08991-8https://doaj.org/toc/2041-1723Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods.Mark A. HerzikMengyu WuGabriel C. LanderNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mark A. Herzik Mengyu Wu Gabriel C. Lander High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
description	Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods.
format	article
author	Mark A. Herzik Mengyu Wu Gabriel C. Lander
author_facet	Mark A. Herzik Mengyu Wu Gabriel C. Lander
author_sort	Mark A. Herzik
title	High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_short	High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_full	High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_fullStr	High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_full_unstemmed	High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_sort	high-resolution structure determination of sub-100 kda complexes using conventional cryo-em
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/082a304c2b0a4f3b94de0ccbd7867848
work_keys_str_mv	AT markaherzik highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem AT mengyuwu highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem AT gabrielclander highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem
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High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM

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