High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM

Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods....

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Autores principales: Mark A. Herzik, Mengyu Wu, Gabriel C. Lander
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/082a304c2b0a4f3b94de0ccbd7867848
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spelling oai:doaj.org-article:082a304c2b0a4f3b94de0ccbd78678482021-12-02T16:58:01ZHigh-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM10.1038/s41467-019-08991-82041-1723https://doaj.org/article/082a304c2b0a4f3b94de0ccbd78678482019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08991-8https://doaj.org/toc/2041-1723Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods.Mark A. HerzikMengyu WuGabriel C. LanderNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Mark A. Herzik
Mengyu Wu
Gabriel C. Lander
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
description Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods.
format article
author Mark A. Herzik
Mengyu Wu
Gabriel C. Lander
author_facet Mark A. Herzik
Mengyu Wu
Gabriel C. Lander
author_sort Mark A. Herzik
title High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_short High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_full High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_fullStr High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_full_unstemmed High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
title_sort high-resolution structure determination of sub-100 kda complexes using conventional cryo-em
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/082a304c2b0a4f3b94de0ccbd7867848
work_keys_str_mv AT markaherzik highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem
AT mengyuwu highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem
AT gabrielclander highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem
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