High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM
Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods....
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Nature Portfolio
2019
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oai:doaj.org-article:082a304c2b0a4f3b94de0ccbd78678482021-12-02T16:58:01ZHigh-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM10.1038/s41467-019-08991-82041-1723https://doaj.org/article/082a304c2b0a4f3b94de0ccbd78678482019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-08991-8https://doaj.org/toc/2041-1723Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods.Mark A. HerzikMengyu WuGabriel C. LanderNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019) |
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Science Q Mark A. Herzik Mengyu Wu Gabriel C. Lander High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM |
description |
Despite many recent advances in cryo-EM, imaging smaller macromolecules (below 100 kDa) has remained a challenge. Here the authors show that biological specimens amassing <100 kDa can be resolved to better than 3 Å resolution using conventional defocus-based single-particle analysis methods. |
format |
article |
author |
Mark A. Herzik Mengyu Wu Gabriel C. Lander |
author_facet |
Mark A. Herzik Mengyu Wu Gabriel C. Lander |
author_sort |
Mark A. Herzik |
title |
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM |
title_short |
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM |
title_full |
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM |
title_fullStr |
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM |
title_full_unstemmed |
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM |
title_sort |
high-resolution structure determination of sub-100 kda complexes using conventional cryo-em |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/082a304c2b0a4f3b94de0ccbd7867848 |
work_keys_str_mv |
AT markaherzik highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem AT mengyuwu highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem AT gabrielclander highresolutionstructuredeterminationofsub100kdacomplexesusingconventionalcryoem |
_version_ |
1718382369154531328 |