A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease
ABSTRACT Marburg virus (MARV) causes disease with high case fatality rates, and there are no approved vaccines or therapies. Licensing of MARV countermeasures will likely require approval via the FDA’s Animal Efficacy Rule, which requires well-characterized animal models that recapitulate human dise...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
American Society for Microbiology
2018
|
| Subjects: | |
| Online Access: | https://doaj.org/article/088abe44dfaf4ff38fcf4dba86cab5a9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:doaj.org-article:088abe44dfaf4ff38fcf4dba86cab5a9 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:088abe44dfaf4ff38fcf4dba86cab5a92021-11-15T15:22:01ZA Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease10.1128/mSphere.00401-172379-5042https://doaj.org/article/088abe44dfaf4ff38fcf4dba86cab5a92018-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00401-17https://doaj.org/toc/2379-5042ABSTRACT Marburg virus (MARV) causes disease with high case fatality rates, and there are no approved vaccines or therapies. Licensing of MARV countermeasures will likely require approval via the FDA’s Animal Efficacy Rule, which requires well-characterized animal models that recapitulate human disease. This includes selection of the virus used for exposure and ensuring that it retains the properties of the original isolate. The consequences of amplification of MARV for challenge studies are unknown. Here, we serially passaged and characterized MARV through 13 passes from the original isolate. Surprisingly, the viral genome was very stable, except for a single nucleotide change that resulted in an amino acid substitution in the hydrophobic region of the signal peptide of the glycoprotein (GP). The particle/PFU ratio also decreased following passages, suggesting a role for the amino acid in viral infectivity. To determine if amplification introduces a phenotype in an animal model, cynomolgus macaques were exposed to either 100 or 0.01 PFU of low- and high-passage-number MARV. All animals succumbed when exposed to 100 PFU of either passage 3 or 13 viruses, although animals exposed to the high-passage-number virus survived longer. However, none of the passage 13 MARV-exposed animals succumbed to 0.01-PFU exposure compared to 75% of passage 3-exposed animals. This is consistent with other filovirus studies that show some particles that are unable to yield a plaque in cell culture can cause lethal disease in vivo. These results have important consequences for the design of experiments that investigate MARV pathogenesis and that test the efficacy of MARV countermeasures. IMPORTANCE Marburg virus (MARV) causes disease with a high case fatality rate, and there are no approved vaccines or therapies. Serial amplification of viruses in cell culture often results in accumulation of mutations, but the effect of such cell culture passage on MARV is unclear. Serial passages of MARV resulted in a single mutation in the region encoding the glycoprotein (GP). This is a region where mutations can have important consequences on outbreaks and human disease [S. Mahanty and M. Bray, Lancet Infect Dis 4:487–498, 2004, https://doi.org/10.1016/S1473-3099(04)01103-X]. We thus investigated whether this mutation impacted disease by using a cynomolgus macaque model of MARV infection. Monkeys exposed to virus containing the mutation had better clinical outcomes than monkeys exposed to virus without the mutation. We also observed that a remarkably low number of MARV particles was sufficient to cause death. Our results could have a significant impact on how future studies are designed to model MARV disease and test vaccines and therapeutics.Kendra J. AlfsonLaura E. AvenaJenny DelgadoMichael W. BeadlesJean L. PattersonRicardo CarrionAnthony GriffithsAmerican Society for MicrobiologyarticleMarburg virusadaptationattenuationglycoproteinmonkey modelpathogenesisMicrobiologyQR1-502ENmSphere, Vol 3, Iss 1 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Marburg virus adaptation attenuation glycoprotein monkey model pathogenesis Microbiology QR1-502 |
| spellingShingle |
Marburg virus adaptation attenuation glycoprotein monkey model pathogenesis Microbiology QR1-502 Kendra J. Alfson Laura E. Avena Jenny Delgado Michael W. Beadles Jean L. Patterson Ricardo Carrion Anthony Griffiths A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease |
| description |
ABSTRACT Marburg virus (MARV) causes disease with high case fatality rates, and there are no approved vaccines or therapies. Licensing of MARV countermeasures will likely require approval via the FDA’s Animal Efficacy Rule, which requires well-characterized animal models that recapitulate human disease. This includes selection of the virus used for exposure and ensuring that it retains the properties of the original isolate. The consequences of amplification of MARV for challenge studies are unknown. Here, we serially passaged and characterized MARV through 13 passes from the original isolate. Surprisingly, the viral genome was very stable, except for a single nucleotide change that resulted in an amino acid substitution in the hydrophobic region of the signal peptide of the glycoprotein (GP). The particle/PFU ratio also decreased following passages, suggesting a role for the amino acid in viral infectivity. To determine if amplification introduces a phenotype in an animal model, cynomolgus macaques were exposed to either 100 or 0.01 PFU of low- and high-passage-number MARV. All animals succumbed when exposed to 100 PFU of either passage 3 or 13 viruses, although animals exposed to the high-passage-number virus survived longer. However, none of the passage 13 MARV-exposed animals succumbed to 0.01-PFU exposure compared to 75% of passage 3-exposed animals. This is consistent with other filovirus studies that show some particles that are unable to yield a plaque in cell culture can cause lethal disease in vivo. These results have important consequences for the design of experiments that investigate MARV pathogenesis and that test the efficacy of MARV countermeasures. IMPORTANCE Marburg virus (MARV) causes disease with a high case fatality rate, and there are no approved vaccines or therapies. Serial amplification of viruses in cell culture often results in accumulation of mutations, but the effect of such cell culture passage on MARV is unclear. Serial passages of MARV resulted in a single mutation in the region encoding the glycoprotein (GP). This is a region where mutations can have important consequences on outbreaks and human disease [S. Mahanty and M. Bray, Lancet Infect Dis 4:487–498, 2004, https://doi.org/10.1016/S1473-3099(04)01103-X]. We thus investigated whether this mutation impacted disease by using a cynomolgus macaque model of MARV infection. Monkeys exposed to virus containing the mutation had better clinical outcomes than monkeys exposed to virus without the mutation. We also observed that a remarkably low number of MARV particles was sufficient to cause death. Our results could have a significant impact on how future studies are designed to model MARV disease and test vaccines and therapeutics. |
| format |
article |
| author |
Kendra J. Alfson Laura E. Avena Jenny Delgado Michael W. Beadles Jean L. Patterson Ricardo Carrion Anthony Griffiths |
| author_facet |
Kendra J. Alfson Laura E. Avena Jenny Delgado Michael W. Beadles Jean L. Patterson Ricardo Carrion Anthony Griffiths |
| author_sort |
Kendra J. Alfson |
| title |
A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease |
| title_short |
A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease |
| title_full |
A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease |
| title_fullStr |
A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease |
| title_full_unstemmed |
A Single Amino Acid Change in the Marburg Virus Glycoprotein Arises during Serial Cell Culture Passages and Attenuates the Virus in a Macaque Model of Disease |
| title_sort |
single amino acid change in the marburg virus glycoprotein arises during serial cell culture passages and attenuates the virus in a macaque model of disease |
| publisher |
American Society for Microbiology |
| publishDate |
2018 |
| url |
https://doaj.org/article/088abe44dfaf4ff38fcf4dba86cab5a9 |
| work_keys_str_mv |
AT kendrajalfson asingleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT lauraeavena asingleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT jennydelgado asingleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT michaelwbeadles asingleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT jeanlpatterson asingleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT ricardocarrion asingleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT anthonygriffiths asingleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT kendrajalfson singleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT lauraeavena singleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT jennydelgado singleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT michaelwbeadles singleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT jeanlpatterson singleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT ricardocarrion singleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease AT anthonygriffiths singleaminoacidchangeinthemarburgvirusglycoproteinarisesduringserialcellculturepassagesandattenuatesthevirusinamacaquemodelofdisease |
| _version_ |
1718428051899940864 |