Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis

Mixed Lineage Kinase Domain-Like (MLKL) pseudokinase is phosphorylated by RIPK3 kinase prior to cell death by necroptosis. Here, the authors use monobodies that bind to the MLKL pseudokinase domain as tools, which allowed them to determine the crystal structures of the MLKL pseudokinase domain in tw...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Sarah E. Garnish, Yanxiang Meng, Akiko Koide, Jarrod J. Sandow, Eric Denbaum, Annette V. Jacobsen, Wayland Yeung, Andre L. Samson, Christopher R. Horne, Cheree Fitzgibbon, Samuel N. Young, Phoebe P. C. Smith, Andrew I. Webb, Emma J. Petrie, Joanne M. Hildebrand, Natarajan Kannan, Peter E. Czabotar, Shohei Koide, James M. Murphy
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Q
Acceso en línea:https://doaj.org/article/09c7c00c35d347a0a64667b29956b651
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:09c7c00c35d347a0a64667b29956b651
record_format dspace
spelling oai:doaj.org-article:09c7c00c35d347a0a64667b29956b6512021-12-02T15:51:06ZConformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis10.1038/s41467-021-22400-z2041-1723https://doaj.org/article/09c7c00c35d347a0a64667b29956b6512021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22400-zhttps://doaj.org/toc/2041-1723Mixed Lineage Kinase Domain-Like (MLKL) pseudokinase is phosphorylated by RIPK3 kinase prior to cell death by necroptosis. Here, the authors use monobodies that bind to the MLKL pseudokinase domain as tools, which allowed them to determine the crystal structures of the MLKL pseudokinase domain in two distinct conformations. By combining their structural data with cell signalling assays and MD simulations they provide evidence that endogenous MLKL preassociates with its upstream regulator RIPK3, and that MLKL disengages from RIPK3 following the induction of necroptosis.Sarah E. GarnishYanxiang MengAkiko KoideJarrod J. SandowEric DenbaumAnnette V. JacobsenWayland YeungAndre L. SamsonChristopher R. HorneCheree FitzgibbonSamuel N. YoungPhoebe P. C. SmithAndrew I. WebbEmma J. PetrieJoanne M. HildebrandNatarajan KannanPeter E. CzabotarShohei KoideJames M. MurphyNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Sarah E. Garnish
Yanxiang Meng
Akiko Koide
Jarrod J. Sandow
Eric Denbaum
Annette V. Jacobsen
Wayland Yeung
Andre L. Samson
Christopher R. Horne
Cheree Fitzgibbon
Samuel N. Young
Phoebe P. C. Smith
Andrew I. Webb
Emma J. Petrie
Joanne M. Hildebrand
Natarajan Kannan
Peter E. Czabotar
Shohei Koide
James M. Murphy
Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
description Mixed Lineage Kinase Domain-Like (MLKL) pseudokinase is phosphorylated by RIPK3 kinase prior to cell death by necroptosis. Here, the authors use monobodies that bind to the MLKL pseudokinase domain as tools, which allowed them to determine the crystal structures of the MLKL pseudokinase domain in two distinct conformations. By combining their structural data with cell signalling assays and MD simulations they provide evidence that endogenous MLKL preassociates with its upstream regulator RIPK3, and that MLKL disengages from RIPK3 following the induction of necroptosis.
format article
author Sarah E. Garnish
Yanxiang Meng
Akiko Koide
Jarrod J. Sandow
Eric Denbaum
Annette V. Jacobsen
Wayland Yeung
Andre L. Samson
Christopher R. Horne
Cheree Fitzgibbon
Samuel N. Young
Phoebe P. C. Smith
Andrew I. Webb
Emma J. Petrie
Joanne M. Hildebrand
Natarajan Kannan
Peter E. Czabotar
Shohei Koide
James M. Murphy
author_facet Sarah E. Garnish
Yanxiang Meng
Akiko Koide
Jarrod J. Sandow
Eric Denbaum
Annette V. Jacobsen
Wayland Yeung
Andre L. Samson
Christopher R. Horne
Cheree Fitzgibbon
Samuel N. Young
Phoebe P. C. Smith
Andrew I. Webb
Emma J. Petrie
Joanne M. Hildebrand
Natarajan Kannan
Peter E. Czabotar
Shohei Koide
James M. Murphy
author_sort Sarah E. Garnish
title Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_short Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_full Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_fullStr Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_full_unstemmed Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_sort conformational interconversion of mlkl and disengagement from ripk3 precede cell death by necroptosis
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/09c7c00c35d347a0a64667b29956b651
work_keys_str_mv AT sarahegarnish conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT yanxiangmeng conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT akikokoide conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT jarrodjsandow conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT ericdenbaum conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT annettevjacobsen conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT waylandyeung conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT andrelsamson conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT christopherrhorne conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT chereefitzgibbon conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT samuelnyoung conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT phoebepcsmith conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT andrewiwebb conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT emmajpetrie conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT joannemhildebrand conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT natarajankannan conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT petereczabotar conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT shoheikoide conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
AT jamesmmurphy conformationalinterconversionofmlklanddisengagementfromripk3precedecelldeathbynecroptosis
_version_ 1718385612738789376