Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis

Mixed Lineage Kinase Domain-Like (MLKL) pseudokinase is phosphorylated by RIPK3 kinase prior to cell death by necroptosis. Here, the authors use monobodies that bind to the MLKL pseudokinase domain as tools, which allowed them to determine the crystal structures of the MLKL pseudokinase domain in tw...

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Autores principales:	Sarah E. Garnish, Yanxiang Meng, Akiko Koide, Jarrod J. Sandow, Eric Denbaum, Annette V. Jacobsen, Wayland Yeung, Andre L. Samson, Christopher R. Horne, Cheree Fitzgibbon, Samuel N. Young, Phoebe P. C. Smith, Andrew I. Webb, Emma J. Petrie, Joanne M. Hildebrand, Natarajan Kannan, Peter E. Czabotar, Shohei Koide, James M. Murphy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/09c7c00c35d347a0a64667b29956b651
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spelling	oai:doaj.org-article:09c7c00c35d347a0a64667b29956b6512021-12-02T15:51:06ZConformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis10.1038/s41467-021-22400-z2041-1723https://doaj.org/article/09c7c00c35d347a0a64667b29956b6512021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22400-zhttps://doaj.org/toc/2041-1723Mixed Lineage Kinase Domain-Like (MLKL) pseudokinase is phosphorylated by RIPK3 kinase prior to cell death by necroptosis. Here, the authors use monobodies that bind to the MLKL pseudokinase domain as tools, which allowed them to determine the crystal structures of the MLKL pseudokinase domain in two distinct conformations. By combining their structural data with cell signalling assays and MD simulations they provide evidence that endogenous MLKL preassociates with its upstream regulator RIPK3, and that MLKL disengages from RIPK3 following the induction of necroptosis.Sarah E. GarnishYanxiang MengAkiko KoideJarrod J. SandowEric DenbaumAnnette V. JacobsenWayland YeungAndre L. SamsonChristopher R. HorneCheree FitzgibbonSamuel N. YoungPhoebe P. C. SmithAndrew I. WebbEmma J. PetrieJoanne M. HildebrandNatarajan KannanPeter E. CzabotarShohei KoideJames M. MurphyNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sarah E. Garnish Yanxiang Meng Akiko Koide Jarrod J. Sandow Eric Denbaum Annette V. Jacobsen Wayland Yeung Andre L. Samson Christopher R. Horne Cheree Fitzgibbon Samuel N. Young Phoebe P. C. Smith Andrew I. Webb Emma J. Petrie Joanne M. Hildebrand Natarajan Kannan Peter E. Czabotar Shohei Koide James M. Murphy Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
description	Mixed Lineage Kinase Domain-Like (MLKL) pseudokinase is phosphorylated by RIPK3 kinase prior to cell death by necroptosis. Here, the authors use monobodies that bind to the MLKL pseudokinase domain as tools, which allowed them to determine the crystal structures of the MLKL pseudokinase domain in two distinct conformations. By combining their structural data with cell signalling assays and MD simulations they provide evidence that endogenous MLKL preassociates with its upstream regulator RIPK3, and that MLKL disengages from RIPK3 following the induction of necroptosis.
format	article
author	Sarah E. Garnish Yanxiang Meng Akiko Koide Jarrod J. Sandow Eric Denbaum Annette V. Jacobsen Wayland Yeung Andre L. Samson Christopher R. Horne Cheree Fitzgibbon Samuel N. Young Phoebe P. C. Smith Andrew I. Webb Emma J. Petrie Joanne M. Hildebrand Natarajan Kannan Peter E. Czabotar Shohei Koide James M. Murphy
author_facet	Sarah E. Garnish Yanxiang Meng Akiko Koide Jarrod J. Sandow Eric Denbaum Annette V. Jacobsen Wayland Yeung Andre L. Samson Christopher R. Horne Cheree Fitzgibbon Samuel N. Young Phoebe P. C. Smith Andrew I. Webb Emma J. Petrie Joanne M. Hildebrand Natarajan Kannan Peter E. Czabotar Shohei Koide James M. Murphy
author_sort	Sarah E. Garnish
title	Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_short	Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_full	Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_fullStr	Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_full_unstemmed	Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis
title_sort	conformational interconversion of mlkl and disengagement from ripk3 precede cell death by necroptosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/09c7c00c35d347a0a64667b29956b651
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Conformational interconversion of MLKL and disengagement from RIPK3 precede cell death by necroptosis

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