Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate
Using photosensitive caged-compound for femtosecond crystallography at X-ray free electron lasers would allow the structure determination of reaction intermediates. Here the authors demonstrate the feasibility of this approach with a caged NO-compound and present the initial NO-bound intermediate st...
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Nature Portfolio
2017
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oai:doaj.org-article:09d185f659f54420bd7260a89d764ebf2021-12-02T14:42:17ZCapturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate10.1038/s41467-017-01702-12041-1723https://doaj.org/article/09d185f659f54420bd7260a89d764ebf2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01702-1https://doaj.org/toc/2041-1723Using photosensitive caged-compound for femtosecond crystallography at X-ray free electron lasers would allow the structure determination of reaction intermediates. Here the authors demonstrate the feasibility of this approach with a caged NO-compound and present the initial NO-bound intermediate structure of cytochrome P450 nitric oxide reductase.Takehiko ToshaTakashi NomuraTakuma NishidaNaoya SaekiKouta OkubayashiRaika YamagiwaMichihiro SugaharaTakanori NakaneKeitaro YamashitaKunio HirataGo UenoTetsunari KimuraTamao HisanoKazumasa MuramotoHitomi SawaiHanae TakedaEiichi MizohataAyumi YamashitaYusuke KanematsuYu TakanoEriko NangoRie TanakaOsamu NurekiOsami ShojiYuka IkemotoHironori MurakamiShigeki OwadaKensuke TonoMakina YabashiMasaki YamamotoHideo AgoSo IwataHiroshi SugimotoYoshitsugu ShiroMinoru KuboNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Takehiko Tosha Takashi Nomura Takuma Nishida Naoya Saeki Kouta Okubayashi Raika Yamagiwa Michihiro Sugahara Takanori Nakane Keitaro Yamashita Kunio Hirata Go Ueno Tetsunari Kimura Tamao Hisano Kazumasa Muramoto Hitomi Sawai Hanae Takeda Eiichi Mizohata Ayumi Yamashita Yusuke Kanematsu Yu Takano Eriko Nango Rie Tanaka Osamu Nureki Osami Shoji Yuka Ikemoto Hironori Murakami Shigeki Owada Kensuke Tono Makina Yabashi Masaki Yamamoto Hideo Ago So Iwata Hiroshi Sugimoto Yoshitsugu Shiro Minoru Kubo Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate |
| description |
Using photosensitive caged-compound for femtosecond crystallography at X-ray free electron lasers would allow the structure determination of reaction intermediates. Here the authors demonstrate the feasibility of this approach with a caged NO-compound and present the initial NO-bound intermediate structure of cytochrome P450 nitric oxide reductase. |
| format |
article |
| author |
Takehiko Tosha Takashi Nomura Takuma Nishida Naoya Saeki Kouta Okubayashi Raika Yamagiwa Michihiro Sugahara Takanori Nakane Keitaro Yamashita Kunio Hirata Go Ueno Tetsunari Kimura Tamao Hisano Kazumasa Muramoto Hitomi Sawai Hanae Takeda Eiichi Mizohata Ayumi Yamashita Yusuke Kanematsu Yu Takano Eriko Nango Rie Tanaka Osamu Nureki Osami Shoji Yuka Ikemoto Hironori Murakami Shigeki Owada Kensuke Tono Makina Yabashi Masaki Yamamoto Hideo Ago So Iwata Hiroshi Sugimoto Yoshitsugu Shiro Minoru Kubo |
| author_facet |
Takehiko Tosha Takashi Nomura Takuma Nishida Naoya Saeki Kouta Okubayashi Raika Yamagiwa Michihiro Sugahara Takanori Nakane Keitaro Yamashita Kunio Hirata Go Ueno Tetsunari Kimura Tamao Hisano Kazumasa Muramoto Hitomi Sawai Hanae Takeda Eiichi Mizohata Ayumi Yamashita Yusuke Kanematsu Yu Takano Eriko Nango Rie Tanaka Osamu Nureki Osami Shoji Yuka Ikemoto Hironori Murakami Shigeki Owada Kensuke Tono Makina Yabashi Masaki Yamamoto Hideo Ago So Iwata Hiroshi Sugimoto Yoshitsugu Shiro Minoru Kubo |
| author_sort |
Takehiko Tosha |
| title |
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate |
| title_short |
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate |
| title_full |
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate |
| title_fullStr |
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate |
| title_full_unstemmed |
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate |
| title_sort |
capturing an initial intermediate during the p450nor enzymatic reaction using time-resolved xfel crystallography and caged-substrate |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/09d185f659f54420bd7260a89d764ebf |
| work_keys_str_mv |
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1718389731713089536 |