Consistent errors in first strand cDNA due to random hexamer mispriming.

Priming of random hexamers in cDNA synthesis is known to show sequence bias, but in addition it has been suggested recently that mismatches in random hexamer priming could be a cause of mismatches between the original RNA fragment and observed sequence reads. To explore random hexamer mispriming as...

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Auteurs principaux: Thomas P van Gurp, Lauren M McIntyre, Koen J F Verhoeven
Format: article
Langue:EN
Publié: Public Library of Science (PLoS) 2013
Sujets:
R
Q
Accès en ligne:https://doaj.org/article/0a0c8dd6777e43fca56e993bbfe3b81e
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