Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia

Preeclampsia (PE), a pregnancy-specific syndrome, is the primary cause of maternal mortality. This work was designed to investigate the specific functions of PTPRO/ ERp44 in the biological behaviors of trophoblast cells and elucidate the underlying molecular mechanism. Constructed siRNA-PTPRO and ER...

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Autores principales:	Yang Yang, Xiaoxia Qiu, Fang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	ptpro erp44 trophoblast cells preeclampsia Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/0a0e1977ffef4fe6a388b4d08d1fe197
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spelling	oai:doaj.org-article:0a0e1977ffef4fe6a388b4d08d1fe1972021-11-04T15:51:54ZProtein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia2165-59792165-598710.1080/21655979.2021.1997561https://doaj.org/article/0a0e1977ffef4fe6a388b4d08d1fe1972021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1997561https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Preeclampsia (PE), a pregnancy-specific syndrome, is the primary cause of maternal mortality. This work was designed to investigate the specific functions of PTPRO/ ERp44 in the biological behaviors of trophoblast cells and elucidate the underlying molecular mechanism. Constructed siRNA-PTPRO and ERp44 overexpression plasmids were transfected into HTR-8/SVneo and JEG-3 cells for further functional experiments. Subsequently, the proliferation and invasion of trophoblast cells were identified by performing CCK-8, flow cytometry and transwell assay. In addition, tube formation assay was employed to estimate the angiogenic ability of HUVECs incubated with the conditioned media (CM) of HTR-8/SVneo or JEG-3 cells. Importantly, the interaction between PTPRO and ERp44 was analyzed through Co-IP. In the current investigation, it was discovered that downregulation of PTPRO notably facilitated the proliferation and invasion of trophoblast cells and induced a stronger in vitro angiogenesis. Moreover, PTPRO interacted with ERp44 to regulate ERp44 expression. ERp44 overexpression suppressed the proliferative, invasive and angiogenic activities of trophoblast cells. As a result, functions of PTPRO knockdown in the biological behaviors of trophoblast cells were partially abrogated upon elevation of ERp44. To sum up, this current research systematically evidenced that PTPRO could regulate the biological behaviors of trophoblast cells by modulating ERp44. Findings may contribute to a novel therapeutic strategy for PE.Yang YangXiaoxia QiuFang WangTaylor & Francis Grouparticleptproerp44trophoblast cellspreeclampsiaBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ptpro erp44 trophoblast cells preeclampsia Biotechnology TP248.13-248.65
spellingShingle	ptpro erp44 trophoblast cells preeclampsia Biotechnology TP248.13-248.65 Yang Yang Xiaoxia Qiu Fang Wang Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia
description	Preeclampsia (PE), a pregnancy-specific syndrome, is the primary cause of maternal mortality. This work was designed to investigate the specific functions of PTPRO/ ERp44 in the biological behaviors of trophoblast cells and elucidate the underlying molecular mechanism. Constructed siRNA-PTPRO and ERp44 overexpression plasmids were transfected into HTR-8/SVneo and JEG-3 cells for further functional experiments. Subsequently, the proliferation and invasion of trophoblast cells were identified by performing CCK-8, flow cytometry and transwell assay. In addition, tube formation assay was employed to estimate the angiogenic ability of HUVECs incubated with the conditioned media (CM) of HTR-8/SVneo or JEG-3 cells. Importantly, the interaction between PTPRO and ERp44 was analyzed through Co-IP. In the current investigation, it was discovered that downregulation of PTPRO notably facilitated the proliferation and invasion of trophoblast cells and induced a stronger in vitro angiogenesis. Moreover, PTPRO interacted with ERp44 to regulate ERp44 expression. ERp44 overexpression suppressed the proliferative, invasive and angiogenic activities of trophoblast cells. As a result, functions of PTPRO knockdown in the biological behaviors of trophoblast cells were partially abrogated upon elevation of ERp44. To sum up, this current research systematically evidenced that PTPRO could regulate the biological behaviors of trophoblast cells by modulating ERp44. Findings may contribute to a novel therapeutic strategy for PE.
format	article
author	Yang Yang Xiaoxia Qiu Fang Wang
author_facet	Yang Yang Xiaoxia Qiu Fang Wang
author_sort	Yang Yang
title	Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia
title_short	Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia
title_full	Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia
title_fullStr	Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia
title_full_unstemmed	Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia
title_sort	protein tyrosine phosphatase receptor type o (ptpro) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing er resident protein 44 (erp44) expression in preeclampsia
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/0a0e1977ffef4fe6a388b4d08d1fe197
work_keys_str_mv	AT yangyang proteintyrosinephosphatasereceptortypeoptproknockdownenhancestheproliferativeinvasiveandangiogenicactivitiesoftrophoblastcellsbysuppressingerresidentprotein44erp44expressioninpreeclampsia AT xiaoxiaqiu proteintyrosinephosphatasereceptortypeoptproknockdownenhancestheproliferativeinvasiveandangiogenicactivitiesoftrophoblastcellsbysuppressingerresidentprotein44erp44expressioninpreeclampsia AT fangwang proteintyrosinephosphatasereceptortypeoptproknockdownenhancestheproliferativeinvasiveandangiogenicactivitiesoftrophoblastcellsbysuppressingerresidentprotein44erp44expressioninpreeclampsia
_version_	1718444693833908224

Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia

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