Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and d...

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Autores principales:	Arturo Carabias, Anders Fuglsang, Piero Temperini, Tillmann Pape, Nicholas Sofos, Stefano Stella, Simon Erlendsson, Guillermo Montoya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0c04ac7b346d45d1bfa7aa86df769b74
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spelling	oai:doaj.org-article:0c04ac7b346d45d1bfa7aa86df769b742021-12-02T17:56:56ZStructure of the mini-RNA-guided endonuclease CRISPR-Cas12j310.1038/s41467-021-24707-32041-1723https://doaj.org/article/0c04ac7b346d45d1bfa7aa86df769b742021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24707-3https://doaj.org/toc/2041-1723The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and discuss Cas12j3 PAM recognition, hybrid stabilisation and the activation mechanism.Arturo CarabiasAnders FuglsangPiero TemperiniTillmann PapeNicholas SofosStefano StellaSimon ErlendssonGuillermo MontoyaNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Arturo Carabias Anders Fuglsang Piero Temperini Tillmann Pape Nicholas Sofos Stefano Stella Simon Erlendsson Guillermo Montoya Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
description	The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and discuss Cas12j3 PAM recognition, hybrid stabilisation and the activation mechanism.
format	article
author	Arturo Carabias Anders Fuglsang Piero Temperini Tillmann Pape Nicholas Sofos Stefano Stella Simon Erlendsson Guillermo Montoya
author_facet	Arturo Carabias Anders Fuglsang Piero Temperini Tillmann Pape Nicholas Sofos Stefano Stella Simon Erlendsson Guillermo Montoya
author_sort	Arturo Carabias
title	Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
title_short	Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
title_full	Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
title_fullStr	Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
title_full_unstemmed	Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
title_sort	structure of the mini-rna-guided endonuclease crispr-cas12j3
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0c04ac7b346d45d1bfa7aa86df769b74
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Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

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