MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.

Acute myeloid leukemia (AML) is as a highly aggressive and heterogeneous hematological malignancy. MiR-20a-5p has been reported to function as an oncogene or tumor suppressor in several tumors, but the clinical significance and regulatory mechanisms of miR-20a-5p in AML cells have not been fully und...

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Autores principales:	Fengchang Bao, Lei Zhang, Xiaohang Pei, Cheng Lian, Yanhui Liu, Hongna Tan, Pingchong Lei
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c417fb8567e4269b36a5b936c5ea5d7
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spelling	oai:doaj.org-article:0c417fb8567e4269b36a5b936c5ea5d72021-12-02T20:14:03ZMiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.1932-620310.1371/journal.pone.0256995https://doaj.org/article/0c417fb8567e4269b36a5b936c5ea5d72021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256995https://doaj.org/toc/1932-6203Acute myeloid leukemia (AML) is as a highly aggressive and heterogeneous hematological malignancy. MiR-20a-5p has been reported to function as an oncogene or tumor suppressor in several tumors, but the clinical significance and regulatory mechanisms of miR-20a-5p in AML cells have not been fully understood. In this study, we found miR-20a-5p was significantly decreased in bone marrow from AML patients, compared with that in healthy controls. Moreover, decreased miR-20a-5p expression was correlated with risk status and poor survival prognosis in AML patients. Overexpression of miR-20a-5p suppressed cell proliferation, induced cell cycle G0/G1 phase arrest and apoptosis in two AML cell lines (THP-1 and U937) using CCK-8 assay and flow cytometry analysis. Moreover, miR-20a-5p overexpression attenuated tumor growth in vivo by performing tumor xenograft experiments. Luciferase reporter assay and western blot demonstrated that protein phosphatase 6 catalytic subunit (PPP6C) as a target gene of miR-20a-5p was negatively regulated by miR-20a-5p in AML cells. Furthermore, PPP6C knockdown imitated, while overexpression reversed the effects of miR-20a-5p overexpression on AML cell proliferation, cell cycle G1/S transition and apoptosis. Taken together, our findings demonstrate that miR-20a-5p/PPP6C represent a new therapeutic target for AML and a potential diagnostic marker for AML therapy.Fengchang BaoLei ZhangXiaohang PeiCheng LianYanhui LiuHongna TanPingchong LeiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256995 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fengchang Bao Lei Zhang Xiaohang Pei Cheng Lian Yanhui Liu Hongna Tan Pingchong Lei MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.
description	Acute myeloid leukemia (AML) is as a highly aggressive and heterogeneous hematological malignancy. MiR-20a-5p has been reported to function as an oncogene or tumor suppressor in several tumors, but the clinical significance and regulatory mechanisms of miR-20a-5p in AML cells have not been fully understood. In this study, we found miR-20a-5p was significantly decreased in bone marrow from AML patients, compared with that in healthy controls. Moreover, decreased miR-20a-5p expression was correlated with risk status and poor survival prognosis in AML patients. Overexpression of miR-20a-5p suppressed cell proliferation, induced cell cycle G0/G1 phase arrest and apoptosis in two AML cell lines (THP-1 and U937) using CCK-8 assay and flow cytometry analysis. Moreover, miR-20a-5p overexpression attenuated tumor growth in vivo by performing tumor xenograft experiments. Luciferase reporter assay and western blot demonstrated that protein phosphatase 6 catalytic subunit (PPP6C) as a target gene of miR-20a-5p was negatively regulated by miR-20a-5p in AML cells. Furthermore, PPP6C knockdown imitated, while overexpression reversed the effects of miR-20a-5p overexpression on AML cell proliferation, cell cycle G1/S transition and apoptosis. Taken together, our findings demonstrate that miR-20a-5p/PPP6C represent a new therapeutic target for AML and a potential diagnostic marker for AML therapy.
format	article
author	Fengchang Bao Lei Zhang Xiaohang Pei Cheng Lian Yanhui Liu Hongna Tan Pingchong Lei
author_facet	Fengchang Bao Lei Zhang Xiaohang Pei Cheng Lian Yanhui Liu Hongna Tan Pingchong Lei
author_sort	Fengchang Bao
title	MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.
title_short	MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.
title_full	MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.
title_fullStr	MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.
title_full_unstemmed	MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.
title_sort	mir-20a-5p functions as a potent tumor suppressor by targeting ppp6c in acute myeloid leukemia.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0c417fb8567e4269b36a5b936c5ea5d7
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MiR-20a-5p functions as a potent tumor suppressor by targeting PPP6C in acute myeloid leukemia.

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