Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Abstract Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptiona...

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Autores principales:	A. F. Lima, G. May, J. Díaz-Colunga, S. Pedreiro, A. Paiva, L. Ferreira, T. Enver, F. J. Iborra, R. Pires das Neves
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0c79bbb7e5ff48728f838a4980fb54fc
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spelling	oai:doaj.org-article:0c79bbb7e5ff48728f838a4980fb54fc2021-12-02T16:08:26ZOsmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation10.1038/s41598-018-25517-22045-2322https://doaj.org/article/0c79bbb7e5ff48728f838a4980fb54fc2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25517-2https://doaj.org/toc/2045-2322Abstract Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34+ umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.A. F. LimaG. MayJ. Díaz-ColungaS. PedreiroA. PaivaL. FerreiraT. EnverF. J. IborraR. Pires das NevesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. F. Lima G. May J. Díaz-Colunga S. Pedreiro A. Paiva L. Ferreira T. Enver F. J. Iborra R. Pires das Neves Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
description	Abstract Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34+ umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.
format	article
author	A. F. Lima G. May J. Díaz-Colunga S. Pedreiro A. Paiva L. Ferreira T. Enver F. J. Iborra R. Pires das Neves
author_facet	A. F. Lima G. May J. Díaz-Colunga S. Pedreiro A. Paiva L. Ferreira T. Enver F. J. Iborra R. Pires das Neves
author_sort	A. F. Lima
title	Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
title_short	Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
title_full	Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
title_fullStr	Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
title_full_unstemmed	Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
title_sort	osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0c79bbb7e5ff48728f838a4980fb54fc
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Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

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