Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.

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Autores principales: Stefan Hans, Daniela Zöller, Juliane Hammer, Johanna Stucke, Sandra Spieß, Gokul Kesavan, Volker Kroehne, Juan Sebastian Eguiguren, Diana Ezhkova, Andreas Petzold, Andreas Dahl, Michael Brand
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Science
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Acceso en línea:https://doaj.org/article/0ca98762369949e99a34f959a12b3002
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https://doaj.org/article/0ca98762369949e99a34f959a12b3002

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