Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Stefan Hans, Daniela Zöller, Juliane Hammer, Johanna Stucke, Sandra Spieß, Gokul Kesavan, Volker Kroehne, Juan Sebastian Eguiguren, Diana Ezhkova, Andreas Petzold, Andreas Dahl, Michael Brand
Format: article
Langue:EN
Publié: Nature Portfolio 2021
Sujets:
Q
Accès en ligne:https://doaj.org/article/0ca98762369949e99a34f959a12b3002
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!