Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.

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Autores principales:	Stefan Hans, Daniela Zöller, Juliane Hammer, Johanna Stucke, Sandra Spieß, Gokul Kesavan, Volker Kroehne, Juan Sebastian Eguiguren, Diana Ezhkova, Andreas Petzold, Andreas Dahl, Michael Brand
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0ca98762369949e99a34f959a12b3002
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spelling	oai:doaj.org-article:0ca98762369949e99a34f959a12b30022021-12-02T14:03:50ZCre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish10.1038/s41467-021-21427-62041-1723https://doaj.org/article/0ca98762369949e99a34f959a12b30022021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21427-6https://doaj.org/toc/2041-1723Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.Stefan HansDaniela ZöllerJuliane HammerJohanna StuckeSandra SpießGokul KesavanVolker KroehneJuan Sebastian EguigurenDiana EzhkovaAndreas PetzoldAndreas DahlMichael BrandNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Stefan Hans Daniela Zöller Juliane Hammer Johanna Stucke Sandra Spieß Gokul Kesavan Volker Kroehne Juan Sebastian Eguiguren Diana Ezhkova Andreas Petzold Andreas Dahl Michael Brand Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
description	Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.
format	article
author	Stefan Hans Daniela Zöller Juliane Hammer Johanna Stucke Sandra Spieß Gokul Kesavan Volker Kroehne Juan Sebastian Eguiguren Diana Ezhkova Andreas Petzold Andreas Dahl Michael Brand
author_facet	Stefan Hans Daniela Zöller Juliane Hammer Johanna Stucke Sandra Spieß Gokul Kesavan Volker Kroehne Juan Sebastian Eguiguren Diana Ezhkova Andreas Petzold Andreas Dahl Michael Brand
author_sort	Stefan Hans
title	Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
title_short	Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
title_full	Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
title_fullStr	Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
title_full_unstemmed	Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
title_sort	cre-controlled crispr mutagenesis provides fast and easy conditional gene inactivation in zebrafish
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0ca98762369949e99a34f959a12b3002
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Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

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