Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish
Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.
Guardado en:
Autores principales: | Stefan Hans, Daniela Zöller, Juliane Hammer, Johanna Stucke, Sandra Spieß, Gokul Kesavan, Volker Kroehne, Juan Sebastian Eguiguren, Diana Ezhkova, Andreas Petzold, Andreas Dahl, Michael Brand |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/0ca98762369949e99a34f959a12b3002 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
aP2-Cre-mediated inactivation of estrogen receptor alpha causes hydrometra.
por: Per Antonson, et al.
Publicado: (2014) -
Deletion of lrrk2 causes early developmental abnormalities and age-dependent increase of monoamine catabolism in the zebrafish brain.
por: Stefano Suzzi, et al.
Publicado: (2021) -
Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration
por: Maira P. Almeida, et al.
Publicado: (2021) -
Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background
por: Lech Kaczmarczyk, et al.
Publicado: (2021) -
Mutagenesis
Publicado: (1986)