Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy
Optical clearing of tissue has enabled optical imaging deeper into tissue due to significantly reduced light scattering. Here, Ryan et al. tackle first-order defocus, an artefact of a non-uniform refractive index, extending light-sheet microscopy to partially cleared samples.
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Autores principales: | Duncan P. Ryan, Elizabeth A. Gould, Gregory J. Seedorf, Omid Masihzadeh, Steven H. Abman, Sukumar Vijayaraghavan, Wendy B. Macklin, Diego Restrepo, Douglas P. Shepherd |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
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Materias: | |
Acceso en línea: | https://doaj.org/article/0ce88ae2961c4eed9544d53128b6b9b0 |
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