Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver

Summary: Nonalcoholic fatty liver disease (NAFLD) is a global health-care problem with limited therapeutic options. To obtain a cellular resolution of pathogenesis, 82,168 single-cell transcriptomes (scRNA-seq) across different NAFLD stages were profiled, identifying hepatocytes and 12 other non-par...

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Autores principales:	Qi Su, Sun Y. Kim, Funmi Adewale, Ye Zhou, Christina Aldler, Min Ni, Yi Wei, Michael E. Burczynski, Gurinder S. Atwal, Mark W. Sleeman, Andrew J. Murphy, Yurong Xin, Xiping Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Animal physiology Cell biology Transcriptomics Science Q
Acceso en línea:	https://doaj.org/article/0cf76087cfa044fd89ddbc08a3f3891a
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spelling	oai:doaj.org-article:0cf76087cfa044fd89ddbc08a3f3891a2021-11-20T05:08:37ZSingle-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver2589-004210.1016/j.isci.2021.103233https://doaj.org/article/0cf76087cfa044fd89ddbc08a3f3891a2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012013https://doaj.org/toc/2589-0042Summary: Nonalcoholic fatty liver disease (NAFLD) is a global health-care problem with limited therapeutic options. To obtain a cellular resolution of pathogenesis, 82,168 single-cell transcriptomes (scRNA-seq) across different NAFLD stages were profiled, identifying hepatocytes and 12 other non-parenchymal cell (NPC) types. scRNA-seq revealed insights into the cellular and molecular mechanisms of the disease. We discovered a dual role for hepatic stellate cells in gene expression regulation and in the potential to trans-differentiate into myofibroblasts. We uncovered distinct expression profiles of Kupffer cells versus monocyte-derived macrophages during NAFLD progression. Kupffer cells showed stronger immune responses, while monocyte-derived macrophages demonstrated a capability for differentiation. Three chimeric NPCs were identified including endothelial-chimeric stellate cells, hepatocyte-chimeric endothelial cells, and endothelial-chimeric Kupffer cells. Our work identified unanticipated aspects of mouse with NAFLD at the single-cell level and advanced the understanding of cellular heterogeneity in NAFLD livers.Qi SuSun Y. KimFunmi AdewaleYe ZhouChristina AldlerMin NiYi WeiMichael E. BurczynskiGurinder S. AtwalMark W. SleemanAndrew J. MurphyYurong XinXiping ChengElsevierarticleAnimal physiologyCell biologyTranscriptomicsScienceQENiScience, Vol 24, Iss 11, Pp 103233- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Animal physiology Cell biology Transcriptomics Science Q
spellingShingle	Animal physiology Cell biology Transcriptomics Science Q Qi Su Sun Y. Kim Funmi Adewale Ye Zhou Christina Aldler Min Ni Yi Wei Michael E. Burczynski Gurinder S. Atwal Mark W. Sleeman Andrew J. Murphy Yurong Xin Xiping Cheng Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver
description	Summary: Nonalcoholic fatty liver disease (NAFLD) is a global health-care problem with limited therapeutic options. To obtain a cellular resolution of pathogenesis, 82,168 single-cell transcriptomes (scRNA-seq) across different NAFLD stages were profiled, identifying hepatocytes and 12 other non-parenchymal cell (NPC) types. scRNA-seq revealed insights into the cellular and molecular mechanisms of the disease. We discovered a dual role for hepatic stellate cells in gene expression regulation and in the potential to trans-differentiate into myofibroblasts. We uncovered distinct expression profiles of Kupffer cells versus monocyte-derived macrophages during NAFLD progression. Kupffer cells showed stronger immune responses, while monocyte-derived macrophages demonstrated a capability for differentiation. Three chimeric NPCs were identified including endothelial-chimeric stellate cells, hepatocyte-chimeric endothelial cells, and endothelial-chimeric Kupffer cells. Our work identified unanticipated aspects of mouse with NAFLD at the single-cell level and advanced the understanding of cellular heterogeneity in NAFLD livers.
format	article
author	Qi Su Sun Y. Kim Funmi Adewale Ye Zhou Christina Aldler Min Ni Yi Wei Michael E. Burczynski Gurinder S. Atwal Mark W. Sleeman Andrew J. Murphy Yurong Xin Xiping Cheng
author_facet	Qi Su Sun Y. Kim Funmi Adewale Ye Zhou Christina Aldler Min Ni Yi Wei Michael E. Burczynski Gurinder S. Atwal Mark W. Sleeman Andrew J. Murphy Yurong Xin Xiping Cheng
author_sort	Qi Su
title	Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver
title_short	Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver
title_full	Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver
title_fullStr	Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver
title_full_unstemmed	Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver
title_sort	single-cell rna transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and nafld mouse liver
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0cf76087cfa044fd89ddbc08a3f3891a
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Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver

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