Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex.
The optimization of antibodies is a desirable goal towards the development of better therapeutic strategies. The antibody 11K2 was previously developed as a therapeutic tool for inflammatory diseases, and displays very high affinity (4.6 pM) for its antigen the chemokine MCP-1 (monocyte chemo-attrac...
Guardado en:
Autores principales: | , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2014
|
Materias: | |
Acceso en línea: | https://doaj.org/article/0cf7920ae8224301853af57115c60f91 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:0cf7920ae8224301853af57115c60f91 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:0cf7920ae8224301853af57115c60f912021-11-18T08:35:36ZAffinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex.1932-620310.1371/journal.pone.0087099https://doaj.org/article/0cf7920ae8224301853af57115c60f912014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24475232/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The optimization of antibodies is a desirable goal towards the development of better therapeutic strategies. The antibody 11K2 was previously developed as a therapeutic tool for inflammatory diseases, and displays very high affinity (4.6 pM) for its antigen the chemokine MCP-1 (monocyte chemo-attractant protein-1). We have employed a virtual library of mutations of 11K2 to identify antibody variants of potentially higher affinity, and to establish benchmarks in the engineering of a mature therapeutic antibody. The most promising candidates identified in the virtual screening were examined by surface plasmon resonance to validate the computational predictions, and to characterize their binding affinity and key thermodynamic properties in detail. Only mutations in the light-chain of the antibody are effective at enhancing its affinity for the antigen in vitro, suggesting that the interaction surface of the heavy-chain (dominated by the hot-spot residue Phe101) is not amenable to optimization. The single-mutation with the highest affinity is L-N31R (4.6-fold higher affinity than wild-type antibody). Importantly, all the single-mutations showing increase affinity incorporate a charged residue (Arg, Asp, or Glu). The characterization of the relevant thermodynamic parameters clarifies the energetic mechanism. Essentially, the formation of new electrostatic interactions early in the binding reaction coordinate (transition state or earlier) benefits the durability of the antibody-antigen complex. The combination of in silico calculations and thermodynamic analysis is an effective strategy to improve the affinity of a matured therapeutic antibody.Masato KiyoshiJose M M CaaveiroEri MiuraSatoru NagatoishiMakoto NakakidoShinji SogaHiroki ShiraiShigeki KawabataKouhei TsumotoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87099 (2014) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Masato Kiyoshi Jose M M Caaveiro Eri Miura Satoru Nagatoishi Makoto Nakakido Shinji Soga Hiroki Shirai Shigeki Kawabata Kouhei Tsumoto Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. |
description |
The optimization of antibodies is a desirable goal towards the development of better therapeutic strategies. The antibody 11K2 was previously developed as a therapeutic tool for inflammatory diseases, and displays very high affinity (4.6 pM) for its antigen the chemokine MCP-1 (monocyte chemo-attractant protein-1). We have employed a virtual library of mutations of 11K2 to identify antibody variants of potentially higher affinity, and to establish benchmarks in the engineering of a mature therapeutic antibody. The most promising candidates identified in the virtual screening were examined by surface plasmon resonance to validate the computational predictions, and to characterize their binding affinity and key thermodynamic properties in detail. Only mutations in the light-chain of the antibody are effective at enhancing its affinity for the antigen in vitro, suggesting that the interaction surface of the heavy-chain (dominated by the hot-spot residue Phe101) is not amenable to optimization. The single-mutation with the highest affinity is L-N31R (4.6-fold higher affinity than wild-type antibody). Importantly, all the single-mutations showing increase affinity incorporate a charged residue (Arg, Asp, or Glu). The characterization of the relevant thermodynamic parameters clarifies the energetic mechanism. Essentially, the formation of new electrostatic interactions early in the binding reaction coordinate (transition state or earlier) benefits the durability of the antibody-antigen complex. The combination of in silico calculations and thermodynamic analysis is an effective strategy to improve the affinity of a matured therapeutic antibody. |
format |
article |
author |
Masato Kiyoshi Jose M M Caaveiro Eri Miura Satoru Nagatoishi Makoto Nakakido Shinji Soga Hiroki Shirai Shigeki Kawabata Kouhei Tsumoto |
author_facet |
Masato Kiyoshi Jose M M Caaveiro Eri Miura Satoru Nagatoishi Makoto Nakakido Shinji Soga Hiroki Shirai Shigeki Kawabata Kouhei Tsumoto |
author_sort |
Masato Kiyoshi |
title |
Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. |
title_short |
Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. |
title_full |
Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. |
title_fullStr |
Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. |
title_full_unstemmed |
Affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. |
title_sort |
affinity improvement of a therapeutic antibody by structure-based computational design: generation of electrostatic interactions in the transition state stabilizes the antibody-antigen complex. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doaj.org/article/0cf7920ae8224301853af57115c60f91 |
work_keys_str_mv |
AT masatokiyoshi affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT josemmcaaveiro affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT erimiura affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT satorunagatoishi affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT makotonakakido affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT shinjisoga affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT hirokishirai affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT shigekikawabata affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex AT kouheitsumoto affinityimprovementofatherapeuticantibodybystructurebasedcomputationaldesigngenerationofelectrostaticinteractionsinthetransitionstatestabilizestheantibodyantigencomplex |
_version_ |
1718421543995834368 |