Defining the genomic signature of totipotency and pluripotency during early human development.

The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (I...

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Main Authors:	Amparo Galan, Patricia Diaz-Gimeno, Maria Eugenia Poo, Diana Valbuena, Eva Sanchez, Veronica Ruiz, Joaquin Dopazo, David Montaner, Ana Conesa, Carlos Simon
Format:	article
Language:	EN
Published:	Public Library of Science (PLoS) 2013
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/0d0e73180e0e49bc948a228200fa8b93
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spelling	oai:doaj.org-article:0d0e73180e0e49bc948a228200fa8b932021-11-18T07:48:58ZDefining the genomic signature of totipotency and pluripotency during early human development.1932-620310.1371/journal.pone.0062135https://doaj.org/article/0d0e73180e0e49bc948a228200fa8b932013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23614026/?tool=EBIhttps://doaj.org/toc/1932-6203The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions.Amparo GalanPatricia Diaz-GimenoMaria Eugenia PooDiana ValbuenaEva SanchezVeronica RuizJoaquin DopazoDavid MontanerAna ConesaCarlos SimonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62135 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amparo Galan Patricia Diaz-Gimeno Maria Eugenia Poo Diana Valbuena Eva Sanchez Veronica Ruiz Joaquin Dopazo David Montaner Ana Conesa Carlos Simon Defining the genomic signature of totipotency and pluripotency during early human development.
description	The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions.
format	article
author	Amparo Galan Patricia Diaz-Gimeno Maria Eugenia Poo Diana Valbuena Eva Sanchez Veronica Ruiz Joaquin Dopazo David Montaner Ana Conesa Carlos Simon
author_facet	Amparo Galan Patricia Diaz-Gimeno Maria Eugenia Poo Diana Valbuena Eva Sanchez Veronica Ruiz Joaquin Dopazo David Montaner Ana Conesa Carlos Simon
author_sort	Amparo Galan
title	Defining the genomic signature of totipotency and pluripotency during early human development.
title_short	Defining the genomic signature of totipotency and pluripotency during early human development.
title_full	Defining the genomic signature of totipotency and pluripotency during early human development.
title_fullStr	Defining the genomic signature of totipotency and pluripotency during early human development.
title_full_unstemmed	Defining the genomic signature of totipotency and pluripotency during early human development.
title_sort	defining the genomic signature of totipotency and pluripotency during early human development.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0d0e73180e0e49bc948a228200fa8b93
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Defining the genomic signature of totipotency and pluripotency during early human development.

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