In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers
Takefumi Narita,1 Shunji Yunoki,1 Yoshimi Ohyabu,1 Naohisa Yahagi,2 Toshio Uraoka3 1Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Koto-ku, 2Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Sh...
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Dove Medical Press
2016
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oai:doaj.org-article:0d6b2b9021104f08979c4973c55ab4052021-12-02T02:40:31ZIn situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers1179-1470https://doaj.org/article/0d6b2b9021104f08979c4973c55ab4052016-12-01T00:00:00Zhttps://www.dovepress.com/in-situ-gelation-properties-of-a-collagenndashgenipin-sol-with-a-poten-peer-reviewed-article-MDERhttps://doaj.org/toc/1179-1470Takefumi Narita,1 Shunji Yunoki,1 Yoshimi Ohyabu,1 Naohisa Yahagi,2 Toshio Uraoka3 1Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Koto-ku, 2Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Shinjuku-ku, 3Department of Gastroenterology, National Hospital Organization Tokyo Medical Center, Meguro-ku, Tokyo, Japan Abstract: We investigated the potential of collagen–genipin sols as biomaterials for treating artificial ulcers following endoscopic submucosal dissection. Collagen sol viscosity increased with condensation, allowing retention on tilted ulcers before gelation and resulting in collagen gel deposition on whole ulcers. The 1.44% collagen sols containing genipin as a crosslinker retained sol fluidity at 23°C for >20 min, facilitating endoscopic use. Collagen sols formed gel depositions on artificial ulcers in response to body temperature, and high temperature responsiveness of gelation because of increased neutral phosphate buffer concentration allowed for thick gel deposition on tilted ulcers. Finally, histological observations showed infiltration of gels into submucosal layers. Taken together, the present data show that genipin-induced crosslinking significantly improves the mechanical properties of collagen gels even at low genipin concentrations of 0.2–1 mM, warranting the use of in situ gelling collagen–genipin sols for endoscopic treatments of gastrointestinal ulcers. Keywords: collagen, genipin, in-situ gel, fibril formation, ulcer dressing Narita TYunoki SOhyabu YYahagi NUraoka TDove Medical Pressarticlecollagengenipinin-situ gelfibril formationulcer dressingMedical technologyR855-855.5ENMedical Devices: Evidence and Research, Vol Volume 9, Pp 429-439 (2016) |
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collagen genipin in-situ gel fibril formation ulcer dressing Medical technology R855-855.5 |
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collagen genipin in-situ gel fibril formation ulcer dressing Medical technology R855-855.5 Narita T Yunoki S Ohyabu Y Yahagi N Uraoka T In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers |
| description |
Takefumi Narita,1 Shunji Yunoki,1 Yoshimi Ohyabu,1 Naohisa Yahagi,2 Toshio Uraoka3 1Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Koto-ku, 2Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Shinjuku-ku, 3Department of Gastroenterology, National Hospital Organization Tokyo Medical Center, Meguro-ku, Tokyo, Japan Abstract: We investigated the potential of collagen–genipin sols as biomaterials for treating artificial ulcers following endoscopic submucosal dissection. Collagen sol viscosity increased with condensation, allowing retention on tilted ulcers before gelation and resulting in collagen gel deposition on whole ulcers. The 1.44% collagen sols containing genipin as a crosslinker retained sol fluidity at 23°C for >20 min, facilitating endoscopic use. Collagen sols formed gel depositions on artificial ulcers in response to body temperature, and high temperature responsiveness of gelation because of increased neutral phosphate buffer concentration allowed for thick gel deposition on tilted ulcers. Finally, histological observations showed infiltration of gels into submucosal layers. Taken together, the present data show that genipin-induced crosslinking significantly improves the mechanical properties of collagen gels even at low genipin concentrations of 0.2–1 mM, warranting the use of in situ gelling collagen–genipin sols for endoscopic treatments of gastrointestinal ulcers. Keywords: collagen, genipin, in-situ gel, fibril formation, ulcer dressing |
| format |
article |
| author |
Narita T Yunoki S Ohyabu Y Yahagi N Uraoka T |
| author_facet |
Narita T Yunoki S Ohyabu Y Yahagi N Uraoka T |
| author_sort |
Narita T |
| title |
In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers |
| title_short |
In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers |
| title_full |
In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers |
| title_fullStr |
In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers |
| title_full_unstemmed |
In situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers |
| title_sort |
in situ gelation properties of a collagen–genipin sol with a potential for the treatment of gastrointestinal ulcers |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/0d6b2b9021104f08979c4973c55ab405 |
| work_keys_str_mv |
AT naritat insitugelationpropertiesofacollagenndashgenipinsolwithapotentialforthetreatmentofgastrointestinalulcers AT yunokis insitugelationpropertiesofacollagenndashgenipinsolwithapotentialforthetreatmentofgastrointestinalulcers AT ohyabuy insitugelationpropertiesofacollagenndashgenipinsolwithapotentialforthetreatmentofgastrointestinalulcers AT yahagin insitugelationpropertiesofacollagenndashgenipinsolwithapotentialforthetreatmentofgastrointestinalulcers AT uraokat insitugelationpropertiesofacollagenndashgenipinsolwithapotentialforthetreatmentofgastrointestinalulcers |
| _version_ |
1718402269223845888 |