Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis

RNA-seq is widely used for transcriptome analysis. Here, the authors analyse a wide spectrum of RNA-seq workflows and present a comprehensive analysis protocol named RNACocktail as well as a computational pipeline leveraging the widely used tools for accurate RNA-seq analysis.

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Autores principales: Sayed Mohammad Ebrahim Sahraeian, Marghoob Mohiyuddin, Robert Sebra, Hagen Tilgner, Pegah T. Afshar, Kin Fai Au, Narges Bani Asadi, Mark B. Gerstein, Wing Hung Wong, Michael P. Snyder, Eric Schadt, Hugo Y. K. Lam
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/0de7f1d5eb3f4b9daae33212abd18a28
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spelling oai:doaj.org-article:0de7f1d5eb3f4b9daae33212abd18a282021-12-02T13:57:55ZGaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis10.1038/s41467-017-00050-42041-1723https://doaj.org/article/0de7f1d5eb3f4b9daae33212abd18a282017-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00050-4https://doaj.org/toc/2041-1723RNA-seq is widely used for transcriptome analysis. Here, the authors analyse a wide spectrum of RNA-seq workflows and present a comprehensive analysis protocol named RNACocktail as well as a computational pipeline leveraging the widely used tools for accurate RNA-seq analysis.Sayed Mohammad Ebrahim SahraeianMarghoob MohiyuddinRobert SebraHagen TilgnerPegah T. AfsharKin Fai AuNarges Bani AsadiMark B. GersteinWing Hung WongMichael P. SnyderEric SchadtHugo Y. K. LamNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Sayed Mohammad Ebrahim Sahraeian
Marghoob Mohiyuddin
Robert Sebra
Hagen Tilgner
Pegah T. Afshar
Kin Fai Au
Narges Bani Asadi
Mark B. Gerstein
Wing Hung Wong
Michael P. Snyder
Eric Schadt
Hugo Y. K. Lam
Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis
description RNA-seq is widely used for transcriptome analysis. Here, the authors analyse a wide spectrum of RNA-seq workflows and present a comprehensive analysis protocol named RNACocktail as well as a computational pipeline leveraging the widely used tools for accurate RNA-seq analysis.
format article
author Sayed Mohammad Ebrahim Sahraeian
Marghoob Mohiyuddin
Robert Sebra
Hagen Tilgner
Pegah T. Afshar
Kin Fai Au
Narges Bani Asadi
Mark B. Gerstein
Wing Hung Wong
Michael P. Snyder
Eric Schadt
Hugo Y. K. Lam
author_facet Sayed Mohammad Ebrahim Sahraeian
Marghoob Mohiyuddin
Robert Sebra
Hagen Tilgner
Pegah T. Afshar
Kin Fai Au
Narges Bani Asadi
Mark B. Gerstein
Wing Hung Wong
Michael P. Snyder
Eric Schadt
Hugo Y. K. Lam
author_sort Sayed Mohammad Ebrahim Sahraeian
title Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis
title_short Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis
title_full Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis
title_fullStr Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis
title_full_unstemmed Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis
title_sort gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum rna-seq analysis
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/0de7f1d5eb3f4b9daae33212abd18a28
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