Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence

The use of probiotic starters can improve the sensory and health-promoting properties of fermented foods. In this study, we developed an anti-inflammatory probiotic starter, Limosilactobacillus reuteri EFEL6901, for use in kimchi fermentation. The EFEL6901 strain was safe for use in foods and was st...

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Autores principales:	Hee Seo, Hyunbin Seong, Ga Yun Kim, Yu Mi Jo, Seong Won Cheon, Youngju Song, Byung Hee Ryu, Hee Kang, Nam Soo Han
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Limosilactobacillus reuteri probiotic kimchi starter inflammatory bowel disease DSS-induced colitis anti-inflammation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0edac61f07804a9490acf528d7ff52cf
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spelling	oai:doaj.org-article:0edac61f07804a9490acf528d7ff52cf2021-12-01T02:23:44ZDevelopment of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence1664-302X10.3389/fmicb.2021.760476https://doaj.org/article/0edac61f07804a9490acf528d7ff52cf2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.760476/fullhttps://doaj.org/toc/1664-302XThe use of probiotic starters can improve the sensory and health-promoting properties of fermented foods. In this study, we developed an anti-inflammatory probiotic starter, Limosilactobacillus reuteri EFEL6901, for use in kimchi fermentation. The EFEL6901 strain was safe for use in foods and was stable under human gastrointestinal conditions. In in vitro experiments, EFEL6901 cells adhered well to colonic epithelial cells and decreased nitric oxide production in lipopolysaccharide-induced macrophages. In in vivo experiments, oral administration of EFEL6901 to DSS-induced colitis mice models significantly alleviated the observed colitis symptoms, prevented body weight loss, lowered the disease activity index score, and prevented colon length shortening. Analysis of these results indicated that EFEL6901 played a probiotic role by preventing the overproduction of pro-inflammatory cytokines, improving gut barrier function, and up-regulating the concentrations of short-chain fatty acids. In addition, EFEL6901 made a fast growth in a simulated kimchi juice and it synthesized similar amounts of metabolites in nabak-kimchi comparable to a commercial kimchi. This study demonstrates that EFEL6901 can be used as a suitable kimchi starter to promote gut health and product quality.Hee SeoHyunbin SeongGa Yun KimYu Mi JoSeong Won CheonYoungju SongByung Hee RyuHee KangNam Soo HanFrontiers Media S.A.articleLimosilactobacillus reuteriprobiotickimchi starterinflammatory bowel diseaseDSS-induced colitisanti-inflammationMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Limosilactobacillus reuteri probiotic kimchi starter inflammatory bowel disease DSS-induced colitis anti-inflammation Microbiology QR1-502
spellingShingle	Limosilactobacillus reuteri probiotic kimchi starter inflammatory bowel disease DSS-induced colitis anti-inflammation Microbiology QR1-502 Hee Seo Hyunbin Seong Ga Yun Kim Yu Mi Jo Seong Won Cheon Youngju Song Byung Hee Ryu Hee Kang Nam Soo Han Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence
description	The use of probiotic starters can improve the sensory and health-promoting properties of fermented foods. In this study, we developed an anti-inflammatory probiotic starter, Limosilactobacillus reuteri EFEL6901, for use in kimchi fermentation. The EFEL6901 strain was safe for use in foods and was stable under human gastrointestinal conditions. In in vitro experiments, EFEL6901 cells adhered well to colonic epithelial cells and decreased nitric oxide production in lipopolysaccharide-induced macrophages. In in vivo experiments, oral administration of EFEL6901 to DSS-induced colitis mice models significantly alleviated the observed colitis symptoms, prevented body weight loss, lowered the disease activity index score, and prevented colon length shortening. Analysis of these results indicated that EFEL6901 played a probiotic role by preventing the overproduction of pro-inflammatory cytokines, improving gut barrier function, and up-regulating the concentrations of short-chain fatty acids. In addition, EFEL6901 made a fast growth in a simulated kimchi juice and it synthesized similar amounts of metabolites in nabak-kimchi comparable to a commercial kimchi. This study demonstrates that EFEL6901 can be used as a suitable kimchi starter to promote gut health and product quality.
format	article
author	Hee Seo Hyunbin Seong Ga Yun Kim Yu Mi Jo Seong Won Cheon Youngju Song Byung Hee Ryu Hee Kang Nam Soo Han
author_facet	Hee Seo Hyunbin Seong Ga Yun Kim Yu Mi Jo Seong Won Cheon Youngju Song Byung Hee Ryu Hee Kang Nam Soo Han
author_sort	Hee Seo
title	Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence
title_short	Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence
title_full	Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence
title_fullStr	Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence
title_full_unstemmed	Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence
title_sort	development of anti-inflammatory probiotic limosilactobacillus reuteri efel6901 as kimchi starter: in vitro and in vivo evidence
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0edac61f07804a9490acf528d7ff52cf
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Development of Anti-inflammatory Probiotic Limosilactobacillus reuteri EFEL6901 as Kimchi Starter: in vitro and In vivo Evidence

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