Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium

This research aims to measure and analyze oxidative stress and ROS, aside from analyzing the detoxification mechanism in the gill of gambusia fish exposed to Cd. During maintenance and acclimatization, fish were fed pellets with smooth water circulation. After seven days of acclimation, the test fis...

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Autores principales:	Adam Moh. Awaludin, Khumaidi Ach., Ramli, Widiastuti Ira Mei, Ernawati, Insivitawati Era, Soegianto Agoes
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/0f4d10940b99486eb880cf30a0d16cfe
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spelling	oai:doaj.org-article:0f4d10940b99486eb880cf30a0d16cfe2021-11-12T11:44:43ZDetoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium2267-124210.1051/e3sconf/202132201025https://doaj.org/article/0f4d10940b99486eb880cf30a0d16cfe2021-01-01T00:00:00Zhttps://www.e3s-conferences.org/articles/e3sconf/pdf/2021/98/e3sconf_isffs2021_01025.pdfhttps://doaj.org/toc/2267-1242This research aims to measure and analyze oxidative stress and ROS, aside from analyzing the detoxification mechanism in the gill of gambusia fish exposed to Cd. During maintenance and acclimatization, fish were fed pellets with smooth water circulation. After seven days of acclimation, the test fish were then transferred to a 4-liter aquarium. Before treatment, fish are fasted for 24 hours to ensure the stomach is empty. The study used 5 treatment groups with 1 control (C), treatment A: 0.03; B: 0.015; C: 0.011; D: 0.007; E: 0.003 (mg/L). The results showed a significant difference between the treatment and control groups (p <0.05). Treatments A and B were showed the highest reactive levels. Stress caused by Cd can increase ROS activity in the gill of gambusia fish. The production of ROS and the exposure to Cd stressors trigger the production of antioxidant enzymes such as catalase (CAT), hydrogen peroxide (H2O2), and superoxide dismutase (SOD). This study concludes that exposure to Cd in juvenile gambusia fish causes oxidative stress through increased antioxidant enzymes and ROS. Analysis related to the mechanism of detoxification of heavy metals in the body of juvenile gambusia fish.Adam Moh. AwaludinKhumaidi Ach.RamliWidiastuti Ira MeiErnawatiInsivitawati EraSoegianto AgoesEDP SciencesarticleEnvironmental sciencesGE1-350ENFRE3S Web of Conferences, Vol 322, p 01025 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Environmental sciences GE1-350
spellingShingle	Environmental sciences GE1-350 Adam Moh. Awaludin Khumaidi Ach. Ramli Widiastuti Ira Mei Ernawati Insivitawati Era Soegianto Agoes Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium
description	This research aims to measure and analyze oxidative stress and ROS, aside from analyzing the detoxification mechanism in the gill of gambusia fish exposed to Cd. During maintenance and acclimatization, fish were fed pellets with smooth water circulation. After seven days of acclimation, the test fish were then transferred to a 4-liter aquarium. Before treatment, fish are fasted for 24 hours to ensure the stomach is empty. The study used 5 treatment groups with 1 control (C), treatment A: 0.03; B: 0.015; C: 0.011; D: 0.007; E: 0.003 (mg/L). The results showed a significant difference between the treatment and control groups (p <0.05). Treatments A and B were showed the highest reactive levels. Stress caused by Cd can increase ROS activity in the gill of gambusia fish. The production of ROS and the exposure to Cd stressors trigger the production of antioxidant enzymes such as catalase (CAT), hydrogen peroxide (H2O2), and superoxide dismutase (SOD). This study concludes that exposure to Cd in juvenile gambusia fish causes oxidative stress through increased antioxidant enzymes and ROS. Analysis related to the mechanism of detoxification of heavy metals in the body of juvenile gambusia fish.
format	article
author	Adam Moh. Awaludin Khumaidi Ach. Ramli Widiastuti Ira Mei Ernawati Insivitawati Era Soegianto Agoes
author_facet	Adam Moh. Awaludin Khumaidi Ach. Ramli Widiastuti Ira Mei Ernawati Insivitawati Era Soegianto Agoes
author_sort	Adam Moh. Awaludin
title	Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium
title_short	Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium
title_full	Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium
title_fullStr	Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium
title_full_unstemmed	Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium
title_sort	detoxification mechanisms in oxidative stress and reactive oxygen species (ros) in gills of gambusia fish (gambusia affinis) exposed to cadmium
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/0f4d10940b99486eb880cf30a0d16cfe
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Detoxification mechanisms in oxidative stress and Reactive Oxygen Species (ROS) in gills of gambusia fish (Gambusia affinis) exposed to Cadmium

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