ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.

Prevalence of ESR1 amplification in breast cancer is highly disputed and discrepancies have been related to different technical protocols and different scoring approaches. In addition, pre-mRNA artifacts have been proposed to influence outcome of ESR1 FISH analysis. We analyzed ESR1 gene copy number...

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Auteurs principaux:	Cathy B Moelans, Frederik Holst, Olaf Hellwinkel, Ronald Simon, Paul J van Diest
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2013
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/0f96661f173f4429996bf67558021dab
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spelling	oai:doaj.org-article:0f96661f173f4429996bf67558021dab2021-11-18T08:41:15ZESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.1932-620310.1371/journal.pone.0084189https://doaj.org/article/0f96661f173f4429996bf67558021dab2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24367641/?tool=EBIhttps://doaj.org/toc/1932-6203Prevalence of ESR1 amplification in breast cancer is highly disputed and discrepancies have been related to different technical protocols and different scoring approaches. In addition, pre-mRNA artifacts have been proposed to influence outcome of ESR1 FISH analysis. We analyzed ESR1 gene copy number status combining an improved RNase FISH protocol with multiplex ligation-dependent probe amplification (MLPA) after laser microdissection. FISH showed a high prevalence of ESR1 gains and amplifications despite RNase treatment but MLPA did not confirm ESR1 copy number increases detected by FISH in more than half of cases. We suggest that the combination of the ESR1-specific intra-tumor heterogeneity and low-level copy number increase accounts for these discrepancies.Cathy B MoelansFrederik HolstOlaf HellwinkelRonald SimonPaul J van DiestPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84189 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cathy B Moelans Frederik Holst Olaf Hellwinkel Ronald Simon Paul J van Diest ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.
description	Prevalence of ESR1 amplification in breast cancer is highly disputed and discrepancies have been related to different technical protocols and different scoring approaches. In addition, pre-mRNA artifacts have been proposed to influence outcome of ESR1 FISH analysis. We analyzed ESR1 gene copy number status combining an improved RNase FISH protocol with multiplex ligation-dependent probe amplification (MLPA) after laser microdissection. FISH showed a high prevalence of ESR1 gains and amplifications despite RNase treatment but MLPA did not confirm ESR1 copy number increases detected by FISH in more than half of cases. We suggest that the combination of the ESR1-specific intra-tumor heterogeneity and low-level copy number increase accounts for these discrepancies.
format	article
author	Cathy B Moelans Frederik Holst Olaf Hellwinkel Ronald Simon Paul J van Diest
author_facet	Cathy B Moelans Frederik Holst Olaf Hellwinkel Ronald Simon Paul J van Diest
author_sort	Cathy B Moelans
title	ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.
title_short	ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.
title_full	ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.
title_fullStr	ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.
title_full_unstemmed	ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.
title_sort	esr1 amplification in breast cancer by optimized rnase fish: frequent but low-level and heterogeneous.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/0f96661f173f4429996bf67558021dab
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ESR1 amplification in breast cancer by optimized RNase FISH: frequent but low-level and heterogeneous.

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