Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast

Abstract Heat stress (HS) is devastating to the poultry industry due to its adverse effects on animal well-being and performance. The effects of heat stress are typically measured using a portable i-STAT blood analyzer that quantifies circulatory hemoglobin concentration and other blood chemistry pa...

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Autores principales:	Sina Dadgar, Elizabeth Greene, Ahmed Dhamad, Barbara Mallmann, Sami Dridi, Narasimhan Rajaram
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11810ecfb263419190095721d305d790
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spelling	oai:doaj.org-article:11810ecfb263419190095721d305d7902021-12-02T14:11:30ZDiffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast10.1038/s41598-021-83293-y2045-2322https://doaj.org/article/11810ecfb263419190095721d305d7902021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83293-yhttps://doaj.org/toc/2045-2322Abstract Heat stress (HS) is devastating to the poultry industry due to its adverse effects on animal well-being and performance. The effects of heat stress are typically measured using a portable i-STAT blood analyzer that quantifies circulatory hemoglobin concentration and other blood chemistry parameters. Here, we used diffuse reflectance spectroscopy (DRS) as a novel non-invasive method to directly determine changes in hematological parameters in the breast tissues of live heat-stressed broilers. Three-week-old male broilers were randomly subjected to two environmental conditions (thermoneutral, TN, 24 °C vs. cyclic heat stress, HS, 35 °C, 12 h/day). Optical spectra were acquired using DRS to monitor breast hemoglobin (Hb) concentration and vascular oxygen saturation (sO2) at three time points: at baseline prior to heat stress, 2 days, and 21 days after initiation of HS. While i-STAT did not demonstrate a discernible change due to HS in circulatory hemoglobin, DRS found a significant decrease in breast Hb and sO2 after exposure to chronic HS. The decrease in sO2 was found to be due to a decrease in oxygenated hemoglobin concentration, indicating a large increase in oxygen consumption in heat-stressed broilers. Our results demonstrate that DRS could potentially be used to study the effects of HS directly in specific organs of interest, such as the breast and thigh, to improve meat quality.Sina DadgarElizabeth GreeneAhmed DhamadBarbara MallmannSami DridiNarasimhan RajaramNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sina Dadgar Elizabeth Greene Ahmed Dhamad Barbara Mallmann Sami Dridi Narasimhan Rajaram Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
description	Abstract Heat stress (HS) is devastating to the poultry industry due to its adverse effects on animal well-being and performance. The effects of heat stress are typically measured using a portable i-STAT blood analyzer that quantifies circulatory hemoglobin concentration and other blood chemistry parameters. Here, we used diffuse reflectance spectroscopy (DRS) as a novel non-invasive method to directly determine changes in hematological parameters in the breast tissues of live heat-stressed broilers. Three-week-old male broilers were randomly subjected to two environmental conditions (thermoneutral, TN, 24 °C vs. cyclic heat stress, HS, 35 °C, 12 h/day). Optical spectra were acquired using DRS to monitor breast hemoglobin (Hb) concentration and vascular oxygen saturation (sO2) at three time points: at baseline prior to heat stress, 2 days, and 21 days after initiation of HS. While i-STAT did not demonstrate a discernible change due to HS in circulatory hemoglobin, DRS found a significant decrease in breast Hb and sO2 after exposure to chronic HS. The decrease in sO2 was found to be due to a decrease in oxygenated hemoglobin concentration, indicating a large increase in oxygen consumption in heat-stressed broilers. Our results demonstrate that DRS could potentially be used to study the effects of HS directly in specific organs of interest, such as the breast and thigh, to improve meat quality.
format	article
author	Sina Dadgar Elizabeth Greene Ahmed Dhamad Barbara Mallmann Sami Dridi Narasimhan Rajaram
author_facet	Sina Dadgar Elizabeth Greene Ahmed Dhamad Barbara Mallmann Sami Dridi Narasimhan Rajaram
author_sort	Sina Dadgar
title	Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
title_short	Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
title_full	Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
title_fullStr	Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
title_full_unstemmed	Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
title_sort	diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/11810ecfb263419190095721d305d790
work_keys_str_mv	AT sinadadgar diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT elizabethgreene diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT ahmeddhamad diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT barbaramallmann diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT samidridi diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast AT narasimhanrajaram diffusereflectancespectroscopyrevealsheatstressinducedchangesinhemoglobinconcentrationinchickenbreast
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Diffuse reflectance spectroscopy reveals heat stress-induced changes in hemoglobin concentration in chicken breast

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