Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α

To assess the effect of Cannabidiol (CBD) on the angiogenesis and stemness of breast cancer cells as well as proliferation. Methods: mRNA level and the amount of protein of vascular endothelial growth factor (VEGF) were determined by qRT-PCR and ELISA. The angiogenic potential of breast cancer cells...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Min Jee Jo, Bu Gyeom Kim, Woo Young Kim, Dae-Hee Lee, Hye Kyeong Yun, Soyeon Jeong, Seong Hye Park, Bo Ram Kim, Jung Lim Kim, Dae Yeong Kim, Sun Il Lee, Sang Cheul Oh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cannabidiol HIF-1α angiogenesis stemness breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/12445ac0a3c34ec1955936b51afebb73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:12445ac0a3c34ec1955936b51afebb73
record_format	dspace
spelling	oai:doaj.org-article:12445ac0a3c34ec1955936b51afebb732021-11-25T17:02:25ZCannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α10.3390/cancers132256672072-6694https://doaj.org/article/12445ac0a3c34ec1955936b51afebb732021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5667https://doaj.org/toc/2072-6694To assess the effect of Cannabidiol (CBD) on the angiogenesis and stemness of breast cancer cells as well as proliferation. Methods: mRNA level and the amount of protein of vascular endothelial growth factor (VEGF) were determined by qRT-PCR and ELISA. The angiogenic potential of breast cancer cells under hypoxic conditions was identified by the HUVEC tube formation assay. The degradation of HIF-1α by CBD and the Src/von Hippel–Lindau tumor suppressor protein (VHL) interaction were assessed by a co-immunoprecipitation assay and Western blotting. To identify the stemness of mamospheres, they were evaluated by the sphere-forming assay and flow cytometry. Results: CBD can suppress angiogenesis and stem cell-like properties of breast cancer through Src/VHL/HIF-1α signaling. CBD may potentially be utilized in the treatment of refractory or recurrent breast cancer.Min Jee JoBu Gyeom KimWoo Young KimDae-Hee LeeHye Kyeong YunSoyeon JeongSeong Hye ParkBo Ram KimJung Lim KimDae Yeong KimSun Il LeeSang Cheul OhMDPI AGarticlecannabidiolHIF-1αangiogenesisstemnessbreast cancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5667, p 5667 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cannabidiol HIF-1α angiogenesis stemness breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	cannabidiol HIF-1α angiogenesis stemness breast cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Min Jee Jo Bu Gyeom Kim Woo Young Kim Dae-Hee Lee Hye Kyeong Yun Soyeon Jeong Seong Hye Park Bo Ram Kim Jung Lim Kim Dae Yeong Kim Sun Il Lee Sang Cheul Oh Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α
description	To assess the effect of Cannabidiol (CBD) on the angiogenesis and stemness of breast cancer cells as well as proliferation. Methods: mRNA level and the amount of protein of vascular endothelial growth factor (VEGF) were determined by qRT-PCR and ELISA. The angiogenic potential of breast cancer cells under hypoxic conditions was identified by the HUVEC tube formation assay. The degradation of HIF-1α by CBD and the Src/von Hippel–Lindau tumor suppressor protein (VHL) interaction were assessed by a co-immunoprecipitation assay and Western blotting. To identify the stemness of mamospheres, they were evaluated by the sphere-forming assay and flow cytometry. Results: CBD can suppress angiogenesis and stem cell-like properties of breast cancer through Src/VHL/HIF-1α signaling. CBD may potentially be utilized in the treatment of refractory or recurrent breast cancer.
format	article
author	Min Jee Jo Bu Gyeom Kim Woo Young Kim Dae-Hee Lee Hye Kyeong Yun Soyeon Jeong Seong Hye Park Bo Ram Kim Jung Lim Kim Dae Yeong Kim Sun Il Lee Sang Cheul Oh
author_facet	Min Jee Jo Bu Gyeom Kim Woo Young Kim Dae-Hee Lee Hye Kyeong Yun Soyeon Jeong Seong Hye Park Bo Ram Kim Jung Lim Kim Dae Yeong Kim Sun Il Lee Sang Cheul Oh
author_sort	Min Jee Jo
title	Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α
title_short	Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α
title_full	Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α
title_fullStr	Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α
title_full_unstemmed	Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α
title_sort	cannabidiol suppresses angiogenesis and stemness of breast cancer cells by downregulation of hypoxia-inducible factors-1α
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/12445ac0a3c34ec1955936b51afebb73
work_keys_str_mv	AT minjeejo cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT bugyeomkim cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT wooyoungkim cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT daeheelee cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT hyekyeongyun cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT soyeonjeong cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT seonghyepark cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT boramkim cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT junglimkim cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT daeyeongkim cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT sunillee cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a AT sangcheuloh cannabidiolsuppressesangiogenesisandstemnessofbreastcancercellsbydownregulationofhypoxiainduciblefactors1a
_version_	1718412758124331008

Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α

Ejemplares similares