Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos

Roughly 25% of mouse genes are embryonically lethal when knocked out, preventing the generation of viable mouse models. Here the authors use CRISPR-Cas9 to edit one blastomere of a two-cell embryo to generate viable chimeric mice.

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Autores principales:	Yi Wu, Jing Zhang, Boya Peng, Dan Tian, Dong Zhang, Yang Li, Xiaoyu Feng, Jinghao Liu, Jun Li, Teng Zhang, Xiaoyong Liu, Jing Lu, Baian Chen, Songlin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/131fc399bebb467090e5dd21ea95996a
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spelling	oai:doaj.org-article:131fc399bebb467090e5dd21ea95996a2021-12-02T15:36:03ZGenerating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos10.1038/s41467-019-10748-22041-1723https://doaj.org/article/131fc399bebb467090e5dd21ea95996a2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10748-2https://doaj.org/toc/2041-1723Roughly 25% of mouse genes are embryonically lethal when knocked out, preventing the generation of viable mouse models. Here the authors use CRISPR-Cas9 to edit one blastomere of a two-cell embryo to generate viable chimeric mice.Yi WuJing ZhangBoya PengDan TianDong ZhangYang LiXiaoyu FengJinghao LiuJun LiTeng ZhangXiaoyong LiuJing LuBaian ChenSonglin WangNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yi Wu Jing Zhang Boya Peng Dan Tian Dong Zhang Yang Li Xiaoyu Feng Jinghao Liu Jun Li Teng Zhang Xiaoyong Liu Jing Lu Baian Chen Songlin Wang Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos
description	Roughly 25% of mouse genes are embryonically lethal when knocked out, preventing the generation of viable mouse models. Here the authors use CRISPR-Cas9 to edit one blastomere of a two-cell embryo to generate viable chimeric mice.
format	article
author	Yi Wu Jing Zhang Boya Peng Dan Tian Dong Zhang Yang Li Xiaoyu Feng Jinghao Liu Jun Li Teng Zhang Xiaoyong Liu Jing Lu Baian Chen Songlin Wang
author_facet	Yi Wu Jing Zhang Boya Peng Dan Tian Dong Zhang Yang Li Xiaoyu Feng Jinghao Liu Jun Li Teng Zhang Xiaoyong Liu Jing Lu Baian Chen Songlin Wang
author_sort	Yi Wu
title	Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos
title_short	Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos
title_full	Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos
title_fullStr	Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos
title_full_unstemmed	Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos
title_sort	generating viable mice with heritable embryonically lethal mutations using the crispr-cas9 system in two-cell embryos
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/131fc399bebb467090e5dd21ea95996a
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Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos

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