A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)

A variety of plants synthesize natural products that either kill or inhibit the growth of various microorganisms. These plant products may serve as useful natural alternatives to synthetic antimicrobial pharmaceuticals and can be especially important in regions where commercial drugs are often not a...

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Autores principales:	Grace J. Miller, Anna M. G. Cunningham, Yui Iwase, Nicole L. Lautensack, W. Matthew Sattley
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/13c0de404ef8487080179e86276d48a4
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spelling	oai:doaj.org-article:13c0de404ef8487080179e86276d48a42021-11-15T15:04:07ZA Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)10.1128/jmbe.v18i3.13061935-78851935-7877https://doaj.org/article/13c0de404ef8487080179e86276d48a42017-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v18i3.1306https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885A variety of plants synthesize natural products that either kill or inhibit the growth of various microorganisms. These plant products may serve as useful natural alternatives to synthetic antimicrobial pharmaceuticals and can be especially important in regions where commercial drugs are often not available. Despite this, the role of plants as producers of natural antimicrobial agents is often understated or even ignored in undergraduate biology curricula. In this laboratory exercise, students extract water-soluble constituents from two plants, Moringa oleifera (moringa) and Allium sativum (garlic), and determine their activity against both a gram-positive (Bacillus cereus strain 971) and a gram-negative (Escherichia coli strain K12) bacterium using a disk diffusion assay on Mueller-Hinton agar. Disks infused with commercially available antibiotics (e.g., penicillin and tetracycline) serve as controls. Following an incubation period of 24 hours, students obtain quantitative data by measuring zones of growth inhibition that develop as a result of strain sensitivity. To determine the effectiveness of the learning objectives, an unannounced quiz was administered both before and after the activity, and the students showed significant gains in their understanding of key concepts. Because this activity combines aspects of two major branches of biology—plant biology and microbiology—it is suitable for use as a laboratory exercise in courses related to either discipline, or it may be used as a laboratory component of a general biology course.Grace J. MillerAnna M. G. CunninghamYui IwaseNicole L. LautensackW. Matthew SattleyAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 18, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Grace J. Miller Anna M. G. Cunningham Yui Iwase Nicole L. Lautensack W. Matthew Sattley A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)
description	A variety of plants synthesize natural products that either kill or inhibit the growth of various microorganisms. These plant products may serve as useful natural alternatives to synthetic antimicrobial pharmaceuticals and can be especially important in regions where commercial drugs are often not available. Despite this, the role of plants as producers of natural antimicrobial agents is often understated or even ignored in undergraduate biology curricula. In this laboratory exercise, students extract water-soluble constituents from two plants, Moringa oleifera (moringa) and Allium sativum (garlic), and determine their activity against both a gram-positive (Bacillus cereus strain 971) and a gram-negative (Escherichia coli strain K12) bacterium using a disk diffusion assay on Mueller-Hinton agar. Disks infused with commercially available antibiotics (e.g., penicillin and tetracycline) serve as controls. Following an incubation period of 24 hours, students obtain quantitative data by measuring zones of growth inhibition that develop as a result of strain sensitivity. To determine the effectiveness of the learning objectives, an unannounced quiz was administered both before and after the activity, and the students showed significant gains in their understanding of key concepts. Because this activity combines aspects of two major branches of biology—plant biology and microbiology—it is suitable for use as a laboratory exercise in courses related to either discipline, or it may be used as a laboratory component of a general biology course.
format	article
author	Grace J. Miller Anna M. G. Cunningham Yui Iwase Nicole L. Lautensack W. Matthew Sattley
author_facet	Grace J. Miller Anna M. G. Cunningham Yui Iwase Nicole L. Lautensack W. Matthew Sattley
author_sort	Grace J. Miller
title	A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)
title_short	A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)
title_full	A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)
title_fullStr	A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)
title_full_unstemmed	A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)
title_sort	laboratory activity demonstrating the antibacterial effects of extracts from two plant species, <italic toggle="yes">moringa oleifera</italic> and <italic toggle="yes">allium sativum</italic> (garlic)
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/13c0de404ef8487080179e86276d48a4
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A Laboratory Activity Demonstrating the Antibacterial Effects of Extracts from Two Plant Species, <italic toggle="yes">Moringa oleifera</italic> and <italic toggle="yes">Allium sativum</italic> (Garlic)

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