4Pi-RESOLFT nanoscopy
The ability to discriminate objects along the optic axis is a benchmark for three-dimensional imaging techniques. Here, the authors combine metastable-state switching and opposing objective lenses to suppress out-of-focus background and record three-dimensional nanoscale images of living cells.
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Main Authors: | Ulrike Böhm, Stefan W. Hell, Roman Schmidt |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2016
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Online Access: | https://doaj.org/article/15612915e17c48a98f956eb5c66c2d2e |
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