Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway

Pyrrolysine (Pyl) exists in nature as the 22nd proteinogenic amino acid, but studies of Pyl have been hindered by the difficulty and inefficiency of both its chemical and biological syntheses. Here, the authors developed an improved PANCE approach to evolve the pylBCD pathway for increased productio...

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Autores principales:	Joanne M. L. Ho, Corwin A. Miller, Kathryn A. Smith, Jacob R. Mattia, Matthew R. Bennett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/15a65cff8dc74dde894fa14065c7385b
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spelling	oai:doaj.org-article:15a65cff8dc74dde894fa14065c7385b2021-12-02T17:14:22ZImproved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway10.1038/s41467-021-24183-92041-1723https://doaj.org/article/15a65cff8dc74dde894fa14065c7385b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24183-9https://doaj.org/toc/2041-1723Pyrrolysine (Pyl) exists in nature as the 22nd proteinogenic amino acid, but studies of Pyl have been hindered by the difficulty and inefficiency of both its chemical and biological syntheses. Here, the authors developed an improved PANCE approach to evolve the pylBCD pathway for increased production of Pyl proteins in E. coli.Joanne M. L. HoCorwin A. MillerKathryn A. SmithJacob R. MattiaMatthew R. BennettNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Joanne M. L. Ho Corwin A. Miller Kathryn A. Smith Jacob R. Mattia Matthew R. Bennett Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway
description	Pyrrolysine (Pyl) exists in nature as the 22nd proteinogenic amino acid, but studies of Pyl have been hindered by the difficulty and inefficiency of both its chemical and biological syntheses. Here, the authors developed an improved PANCE approach to evolve the pylBCD pathway for increased production of Pyl proteins in E. coli.
format	article
author	Joanne M. L. Ho Corwin A. Miller Kathryn A. Smith Jacob R. Mattia Matthew R. Bennett
author_facet	Joanne M. L. Ho Corwin A. Miller Kathryn A. Smith Jacob R. Mattia Matthew R. Bennett
author_sort	Joanne M. L. Ho
title	Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway
title_short	Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway
title_full	Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway
title_fullStr	Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway
title_full_unstemmed	Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway
title_sort	improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/15a65cff8dc74dde894fa14065c7385b
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Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway

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