A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy

Electron microscopy (EM) is the gold standard for biological ultrastructure but acquisition speed is slow, making it unsuitable for large volumes. Here the authors present a parallel imaging pipeline for continuous autonomous imaging with six transmission EMs to image 1 mm3 of mouse cortex in less t...

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Autores principales:	Wenjing Yin, Derrick Brittain, Jay Borseth, Marie E. Scott, Derric Williams, Jedediah Perkins, Christopher S. Own, Matthew Murfitt, Russel M. Torres, Daniel Kapner, Gayathri Mahalingam, Adam Bleckert, Daniel Castelli, David Reid, Wei-Chung Allen Lee, Brett J. Graham, Marc Takeno, Daniel J. Bumbarger, Colin Farrell, R. Clay Reid, Nuno Macarico da Costa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1636c301c307427c8f6136be5b12c53a
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spelling	oai:doaj.org-article:1636c301c307427c8f6136be5b12c53a2021-12-02T18:51:23ZA petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy10.1038/s41467-020-18659-32041-1723https://doaj.org/article/1636c301c307427c8f6136be5b12c53a2020-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18659-3https://doaj.org/toc/2041-1723Electron microscopy (EM) is the gold standard for biological ultrastructure but acquisition speed is slow, making it unsuitable for large volumes. Here the authors present a parallel imaging pipeline for continuous autonomous imaging with six transmission EMs to image 1 mm3 of mouse cortex in less than 6 months.Wenjing YinDerrick BrittainJay BorsethMarie E. ScottDerric WilliamsJedediah PerkinsChristopher S. OwnMatthew MurfittRussel M. TorresDaniel KapnerGayathri MahalingamAdam BleckertDaniel CastelliDavid ReidWei-Chung Allen LeeBrett J. GrahamMarc TakenoDaniel J. BumbargerColin FarrellR. Clay ReidNuno Macarico da CostaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wenjing Yin Derrick Brittain Jay Borseth Marie E. Scott Derric Williams Jedediah Perkins Christopher S. Own Matthew Murfitt Russel M. Torres Daniel Kapner Gayathri Mahalingam Adam Bleckert Daniel Castelli David Reid Wei-Chung Allen Lee Brett J. Graham Marc Takeno Daniel J. Bumbarger Colin Farrell R. Clay Reid Nuno Macarico da Costa A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
description	Electron microscopy (EM) is the gold standard for biological ultrastructure but acquisition speed is slow, making it unsuitable for large volumes. Here the authors present a parallel imaging pipeline for continuous autonomous imaging with six transmission EMs to image 1 mm3 of mouse cortex in less than 6 months.
format	article
author	Wenjing Yin Derrick Brittain Jay Borseth Marie E. Scott Derric Williams Jedediah Perkins Christopher S. Own Matthew Murfitt Russel M. Torres Daniel Kapner Gayathri Mahalingam Adam Bleckert Daniel Castelli David Reid Wei-Chung Allen Lee Brett J. Graham Marc Takeno Daniel J. Bumbarger Colin Farrell R. Clay Reid Nuno Macarico da Costa
author_facet	Wenjing Yin Derrick Brittain Jay Borseth Marie E. Scott Derric Williams Jedediah Perkins Christopher S. Own Matthew Murfitt Russel M. Torres Daniel Kapner Gayathri Mahalingam Adam Bleckert Daniel Castelli David Reid Wei-Chung Allen Lee Brett J. Graham Marc Takeno Daniel J. Bumbarger Colin Farrell R. Clay Reid Nuno Macarico da Costa
author_sort	Wenjing Yin
title	A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
title_short	A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
title_full	A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
title_fullStr	A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
title_full_unstemmed	A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
title_sort	petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/1636c301c307427c8f6136be5b12c53a
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A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy

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