Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells.
c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering assoc...
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2011
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oai:doaj.org-article:16b8c885354a4927afa00f7e70e812142021-11-18T06:58:17ZSpecific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells.1932-620310.1371/journal.pone.0017237https://doaj.org/article/16b8c885354a4927afa00f7e70e812142011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21390316/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering associated with a localisation of β-catenin at cell membranes and a reduction of expression of β-catenin target genes. c-Yes silencing induced an increase in apoptosis, inhibition of growth in soft-agar and in mouse xenografts, inhibition of cell migration and loss of the capacity to generate liver metastases in mice. Re-introduction of c-Yes, but not c -Src, restores transforming properties of c-Yes depleted cells. Moreover, we found that c-Yes kinase activity is required for its role in β-catenin localisation and growth in soft agar, whereas kinase activity is dispensable for its role in cell migration. We conclude that c-Yes regulates specific oncogenic signalling pathways important for colon cancer progression that is not shared with c-Src.Florence SancierAurélie DumontAudrey SirventLudmilla Paquay de PlaterThomas EdmondsGéraldine DavidMichel JanCatherine de MontrionFrancis CogéStéphane LéonceMichael BurbridgeAlain BrunoJean A BoutinBrian LockhartSerge RocheFrancisco CruzaleguiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17237 (2011) |
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Medicine R Science Q Florence Sancier Aurélie Dumont Audrey Sirvent Ludmilla Paquay de Plater Thomas Edmonds Géraldine David Michel Jan Catherine de Montrion Francis Cogé Stéphane Léonce Michael Burbridge Alain Bruno Jean A Boutin Brian Lockhart Serge Roche Francisco Cruzalegui Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells. |
| description |
c-Yes, a member of the Src tyrosine kinase family, is found highly activated in colon carcinoma but its importance relative to c-Src has remained unclear. Here we show that, in HT29 colon carcinoma cells, silencing of c-Yes, but not of c-Src, selectively leads to an increase of cell clustering associated with a localisation of β-catenin at cell membranes and a reduction of expression of β-catenin target genes. c-Yes silencing induced an increase in apoptosis, inhibition of growth in soft-agar and in mouse xenografts, inhibition of cell migration and loss of the capacity to generate liver metastases in mice. Re-introduction of c-Yes, but not c -Src, restores transforming properties of c-Yes depleted cells. Moreover, we found that c-Yes kinase activity is required for its role in β-catenin localisation and growth in soft agar, whereas kinase activity is dispensable for its role in cell migration. We conclude that c-Yes regulates specific oncogenic signalling pathways important for colon cancer progression that is not shared with c-Src. |
| format |
article |
| author |
Florence Sancier Aurélie Dumont Audrey Sirvent Ludmilla Paquay de Plater Thomas Edmonds Géraldine David Michel Jan Catherine de Montrion Francis Cogé Stéphane Léonce Michael Burbridge Alain Bruno Jean A Boutin Brian Lockhart Serge Roche Francisco Cruzalegui |
| author_facet |
Florence Sancier Aurélie Dumont Audrey Sirvent Ludmilla Paquay de Plater Thomas Edmonds Géraldine David Michel Jan Catherine de Montrion Francis Cogé Stéphane Léonce Michael Burbridge Alain Bruno Jean A Boutin Brian Lockhart Serge Roche Francisco Cruzalegui |
| author_sort |
Florence Sancier |
| title |
Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells. |
| title_short |
Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells. |
| title_full |
Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells. |
| title_fullStr |
Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells. |
| title_full_unstemmed |
Specific oncogenic activity of the Src-family tyrosine kinase c-Yes in colon carcinoma cells. |
| title_sort |
specific oncogenic activity of the src-family tyrosine kinase c-yes in colon carcinoma cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/16b8c885354a4927afa00f7e70e81214 |
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