Evaluation of carboxyfluorescein-labeled 7-methylguanine nucleotides as probes for studying cap-binding proteins by fluorescence anisotropy

Abstract Fluorescence anisotropy (FA) is a powerful technique for the discovery of protein inhibitors in a high-throughput manner. In this study, we sought to develop new universal FA-based assays for the evaluation of compounds targeting mRNA 5′ cap-binding proteins of therapeutic interest, includi...

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Auteurs principaux: Anna Wojtczak, Renata Kasprzyk, Marcin Warmiński, Krystian Ubych, Dorota Kubacka, Pawel J. Sikorski, Jacek Jemielity, Joanna Kowalska
Format: article
Langue:EN
Publié: Nature Portfolio 2021
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Accès en ligne:https://doaj.org/article/17b379a52296481f8cd837d1cb2a8f0d
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